Current Strategies to Improve Yield of Recombinant Protein Production in Rice Suspension Cells

A plant cell-based recombinant glucocerebrosidase was approved by the FDA in 2012 for the treatment of human inherited Gaucher disease, indicating that plant suspension cells have advantages in biosafety and a low production cost as a commercial pharmaceutical recombinant protein expression system. A low allergenic rice suspension cell-based recombinant protein expression system controlled by the alpha Amy3/RAmy3D promoter has been shown to result in relatively high protein yields in plant cell-based systems. Although several recombinant proteins have been produced in rice suspension cell-based systems, yields must be improved to compete with the current commercial protein expression systems. Different strategies were performed and showed successful improvements in recombinant protein yields in this rice system. The review updates and highlights strategies for potential improvements of the alpha Amy3-based rice suspension cell-based system.

Automated summary

A plant cell-based recombinant protein expression system has advantages in biosafety and cost, but its yields need to be improved. This review updates and highlights potential strategies to improve the alpha Amy3-based rice suspension cell-based system.