期刊
PROCESSES
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/pr10051037
关键词
genetically modified soybean and hot pepper; soil microbial community structures; real-time PCR-DGGE; NGS; persistence of transgenes
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF2018R1D1A1B07047456]
This study assessed the impact of genetically modified crops on soil microbial community structures and found that there was no significant difference between GM and non-GM crops. The level of the transgene in GM soybean plots increased during active growth and then gradually decreased, while in GM hot pepper plots it fluctuated according to the flowering stages.
With the advance in gene technology, genetically modified (GM) crops have increased in recent years. GM crops offer us various benefits. However, there are potential risks of GM crops for the environment. In this study, the impacts of transgenic plants on soil microbial community structures were assessed. Two varieties of soybean (Glycine max L.) and hot pepper (Capsicum annuum L.), which introduced the herbicide-resistant gene, bar, were used in this study. The effects of GM crops on soil microbial community structures were investigated using a cultural method, the denaturing gradient gel electrophoresis (DGGE) procedure, and 16S rRNA gene sequencing on the Illumina platform. Additionally, the persistence of transgenes was monitored using a quantitative real-time PCR procedure. The cultural method, DGGE analysis, and the amplicon-based community profile indicated that soil microbial communities were not significantly different between GM and non-GM lines. The level of the bar gene in GM soybean plots greatly increased when the crops were actively growing, but thereafter gradually decreased to the initial level. Meanwhile, the level of the bar gene in GM hot pepper plots repeatedly increased and decreased according to the flowering stages. These results indicated that soil microbial community structures were not significantly affected.
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