期刊
PROCESSES
卷 10, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/pr10050873
关键词
biodegradation; PAH; Aspergillus glaucus; phenol 2-monooxygenase; catechol 1,2-dioxygenase
资金
- Operational programme Science and Education for Smart Growth [BG05M2OP001-1.002-0019]
- European Union through the European structural and investment funds
This study provides original data on the potential of Antarctic fungal strain Aspergillus glaucus AL1 to degrade naphthalene and anthracene, identifying key intermediates and enzyme activities in the process.
Biotechnologies based on microbial species capable of destroying harmful pollutants are a successful way to solve some of the most important problems associated with a clean environment. The subject of investigation is the Antarctic fungal strain Aspergillus glaucus AL1. The culturing of the examined strain was performed with 70 mg of wet mycelium being inoculated in a Czapek Dox liquid medium containing naphthalene, anthracene, or phenanthrene (0.3 g/L) as the sole carbon source. Progressively decreasing naphthalene and anthracene concentrations were monitored in the culture medium until the 15th day of the cultivation of A. glaucus AL1. The degradation was determined through gas chromatography-mass spectrometry. Both decreased by 66% and 44%, respectively, for this period. The GC-MS analyses were applied to identify salicylic acid, catechol, and ketoadipic acid as intermediates in the naphthalene degradation. The intermediates identified in anthracene catabolism are 2-hydroxy-1-naphthoic acid, o-phthalic acid, and protocatechuic acid. The enzyme activities for phenol 2-monooxygenase (1.14.13.7) and catechol 1,2-dioxygenase (1.13.11.1) were established. A gene encoding an enzyme with catechol 1,2-dioxygenase activity was identified and sequenced (GeneBank Ac. No KM360483). The recent study provides original data on the potential of an ascomycete's fungal strain A. glaucus strain AL 1 to degrade naphthalene and anthracene.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据