期刊
PHARMACEUTICS
卷 14, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/pharmaceutics14030594
关键词
CXCL16; ADAM10; NF-kappa beta; apoptosis; pancreatic islets; resveratrol; T1D
资金
- Qassim University [10103-pharmacy2020-1-3-1]
This study discovered the critical role of ADAM10 and CXCL16 in the development of T1D, and the protective effect of resveratrol (RES) in preventing the deleterious effects caused by STZ. ADAM10 and CXCL16 may serve as potential therapeutic targets for T1D.
Background: CXCL16 attracts T-cells to the site of inflammation after cleaving by A Disintegrin and Metalloproteinase (ADAM10). Aim: The current study explored the role of ADAM10/CXCL16/Tcell/NF-kappa B in the initiation of type 1 diabetes (T1D) with special reference to the potential protecting role of resveratrol (RES). Methods: Four sets of Balb/c mice were created: a diabetes mellitus (DM) group (streptozotocin (STZ) 55 mg/kg, i.p.], a control group administered buffer, a RES group [RES, 50 mg/kg, i.p.), and a DM + RES group (RES (50 mg/kg, i.p.) and STZ (55 mg/kg, i.p.) administered daily for 12 days commencing from the fourth day of STZ injection). Histopathological changes, fasting blood insulin (FBI), glucose (FBG), serum and pancreatic ADAM10, CXCL16, NF-kappa B, T-cells pancreatic expression, inflammatory, and apoptotic markers were analyzed. Results: FBG, inflammatory and apoptotic markers, serum TNF-alpha, cellular CXCL16 and ADAM10 protein expression, pancreatic T-cell migration and NF-kappa B were significantly increased in diabetic mice compared to normal mice. RES significantly improved the biochemical and inflammatory parameters distorted in STZ-treated mice. Conclusions: ADAM10 promotes the cleaved form of CXCL16 driving T-cells into the islets of the pancreatic in T1D. RES successfully prevented the deleterious effect caused by STZ. ADAM10 and CXCL16 may serve as novel therapeutic targets for T1D.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据