期刊
CELLS
卷 11, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/cells11091519
关键词
mitotic exit network; septation initiation network; Ogataea polymorpha; Cdc15 kinase; SPB; Cdc5 kinase
类别
资金
- JSPS [24570214, JP19K06641]
- Initiative for Realizing Diversity in the Research Environment
- Institute for Fermentation Osaka
- Noda Institute for Scientific Research
- German Research Council Deutsche Forschungsgemeinschaft, DFG [PE1883-1/2/3, SFB873, A14]
- Grants-in-Aid for Scientific Research [24570214] Funding Source: KAKEN
The mitotic exit network (MEN) is important for terminating mitosis in yeasts, but its molecular mechanisms differ between different species.
The mitotic exit network (MEN) is a conserved signalling pathway essential for the termination of mitosis in the budding yeast Saccharomyces cerevisiae. All MEN components are highly conserved in the methylotrophic budding yeast Ogataea polymorpha, except for Cdc15 kinase. Instead, we identified two essential kinases OpHcd1 and OpHcd2 (homologue candidate of ScCdc15) that are homologous to SpSid1 and SpCdc7, respectively, components of the septation initiation network (SIN) of the fission yeast Schizosaccharomyces pombe. Conditional mutants for OpHCD1 and OpHCD2 exhibited significant delay in late anaphase and defective cell separation, suggesting that both genes have roles in mitotic exit and cytokinesis. Unlike Cdc15 in S. cerevisiae, the association of OpHcd1 and OpHcd2 with the yeast centrosomes (named spindle pole bodies, SPBs) is restricted to the SPB in the mother cell body. SPB localisation of OpHcd2 is regulated by the status of OpTem1 GTPase, while OpHcd1 requires the polo-like kinase OpCdc5 as well as active Tem1 to ensure the coordination of mitotic exit (ME) signalling and cell cycle progression. Our study suggests that the divergence of molecular mechanisms to control the ME-signalling pathway as well as the loss of Sid1/Hcd1 kinase in the MEN occurred relatively recently during the evolution of budding yeast.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据