期刊
CELLS
卷 11, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/cells11101629
关键词
cytoskeleton; human adipose-derived stem cells; cytochalasin B; actin microfilaments; atomic force microscopy; nanomechanics; adipogenic commitment
类别
资金
- Di Bella Foundation, Via Guglielmo Marconi Bologna, Italy
- Eldor Lab, via Vittor Pisani Milan, Italy
Cytoskeletal proteins play a crucial role in providing architectural and signaling cues within cells, influencing cell shape, proliferation, and differentiation. Changes in cytoskeletal proteins modulate the viscoelastic properties of cells, thereby affecting their fate in adipogenesis.
Cytoskeletal proteins provide architectural and signaling cues within cells. They are able to reorganize themselves in response to mechanical forces, converting the stimuli received into specific cellular responses. Thus, the cytoskeleton influences cell shape, proliferation, and even differentiation. In particular, the cytoskeleton affects the fate of mesenchymal stem cells (MSCs), which are highly attractive candidates for cell therapy approaches due to their capacity for self-renewal and multi-lineage differentiation. Cytochalasin B (CB), a cyto-permeable mycotoxin, is able to inhibit the formation of actin microfilaments, resulting in direct effects on cell biological properties. Here, we investigated for the first time the effects of different concentrations of CB (0.1-10 mu M) on human adipose-derived stem cells (hASCs) both after 24 h (h) of CB treatment and 24 h after CB wash-out. CB influenced the metabolism, proliferation, and morphology of hASCs in a dose-dependent manner, in association with progressive disorganization of actin microfilaments. Furthermore, the removal of CB highlighted the ability of cells to restore their cytoskeletal organization. Finally, atomic force microscopy (AFM) revealed that cytoskeletal changes induced by CB modulated the viscoelastic properties of hASCs, influencing their stiffness and viscosity, thereby affecting adipogenic fate.
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