期刊
CELLS
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/cells11081313
关键词
mesenchymal stem; stromal cells; 2D culture; 3D culture; spheroids; adipogenic differentiation; adipokines
类别
资金
- Federal State of Mecklenburg-Vorpommern (ARENA) [TBI-V-003-VBU-001]
- Research Fund of the Department of Cell Biology, Rostock University Medical Center
The cultivation conditions are crucial for the behavior of adMSC cells, as adipogenic differentiation only occurs under specific stimulation. Compared to 2D culture, the 3D culture environment has significant effects on adipogenic differentiation and adipokine release.
Mesenchymal stem/stromal cells (MSC) are capable of renewing the progenitor cell fraction or differentiating in a tissue-specific manner. Adipogenic differentiation of adipose-tissue-derived MSC (adMSC) is important in various pathological processes. Adipocytes and their progenitors are metabolically active and secrete molecules (adipokines) that have both pro- and anti-inflammatory properties. Cell culturing in 2D is commonly used to study cellular responses, but the 2D environment does not reflect the structural situation for most cell types. Therefore, 3D culture systems have been developed to create an environment considered more physiological. Since knowledge about the effects of 3D cultivation on adipogenic differentiation is limited, we investigated its effects on adipogenic differentiation and adipokine release of adMSC (up to 28 days) and compared these with the effects in 2D. We demonstrated that cultivation conditions are crucial for cell behavior: in both 2D and 3D culture, adipogenic differentiation occurred only after specific stimulation. While the size and structure of adipogenically stimulated 3D spheroids remained stable during the experiment, the unstimulated spheroids showed signs of disintegration. Adipokine release was dependent on culture dimensionality; we found upregulated adiponectin and downregulated pro-inflammatory factors. Our findings are relevant for cell therapeutic applications of adMSC in complex, three-dimensionally arranged tissues.
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