期刊
CELLS
卷 11, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/cells11091562
关键词
allogeneic serum; excluded volume effect; foetal bovine serum; serial passaging; tenocyte aging; tenocyte function
类别
资金
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie [676338]
- European Research Council (ERC) under the European Union [866126]
- Twinning Widespread Coordination and Support Action [810850]
- Science Foundation Ireland (SFI)
- European Regional Development Fund (ERDF) [13/RC/2073_2]
- European Research Council (ERC) [866126] Funding Source: European Research Council (ERC)
The use of xenogeneic sera in in vitro culture can lead to rapid loss of tenocyte function. Our study demonstrates that using allogeneic sera and tissue-specific extracellular matrix can effectively expand equine tenocytes.
The absence of a native extracellular matrix and the use of xenogeneic sera are often associated with rapid tenocyte function losses during in vitro culture. Herein, we assessed the influence of different sera (equine serum and foetal bovine serum) on equine tenocyte morphology, viability, metabolic activity, proliferation and protein synthesis as a function of tissue-specific extracellular matrix deposition (induced via macromolecular crowding), aging (passages 3, 6, 9) and time in culture (days 3, 5, 7). In comparison to cells at passage 3, at day 3, in foetal bovine serum and without macromolecular crowding (traditional equine tenocyte culture), the highest number of significantly decreased readouts were observed for cells in foetal bovine serum, at passage 3, at day 5 and day 7 and without macromolecular crowding. Again, in comparison to traditional equine tenocyte culture, the highest number of significantly increased readouts were observed for cells in equine serum, at passage 3 and passage 6, at day 7 and with macromolecular crowding. Our data advocate the use of an allogeneic serum and tissue-specific extracellular matrix for effective expansion of equine tenocytes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据