期刊
CELLS
卷 11, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/cells11081258
关键词
human iPSCs; decellularization; organ-derived scaffold; extracellular matrix; microminiature pig; xeno-implantation; xeno-transplantation
类别
资金
- Japan Agency for Medical Research and Development (AMED) [20bm0404038h0003, 21bm1004003h0002]
Human induced pluripotent stem cells (hiPSCs) are a promising cell source for studying disease pathology and treatment. In this study, hiPSC-derived cells were successfully seeded onto decellularized organ-derived scaffolds and evaluated in an in vivo-like environment.
Human induced pluripotent stem cells (hiPSCs) are a promising cell source for elucidating disease pathology and therapy. The mass supply of hiPSC-derived cells is technically feasible. Carriers that can contain a large number of hiPSC-derived cells and evaluate their functions in vivo-like environments will become increasingly important for understanding disease pathogenesis or treating end-stage organ failure. hiPSC-derived hepatocyte-like cells (hiPSC-HLCs; 5 x 10(8)) were seeded into decellularized organ-derived scaffolds under circumfusion culture. The scaffolds were implanted into immunodeficient microminiature pigs to examine their applicability in vivo. The seeded hiPSC-HLCs demonstrated increased albumin secretion and up-regulated cytochrome P450 activities compared with those in standard two-dimensional culture conditions. Moreover, they showed long-term survival accompanied by neovascularization in vivo. The decellularized organ-derived scaffold is a promising carrier for hiPSC-derived cells for ex vivo and in vivo use and is an essential platform for regenerative medicine and research.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据