期刊
ACTA PHARMACEUTICA SINICA B
卷 12, 期 11, 页码 4235-4248出版社
INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
DOI: 10.1016/j.apsb.2022.03.015
关键词
Erythroliposome; Broad-spectrum detoxification; Hybrid nanovesicle; Pore-forming toxins; Macrophage uptake; Red blood cell membrane; Artificial lipid membrane
资金
- National Natural Science Foundation of China [81773283]
Anti-virulence strategy is a promising approach to combat drug-resistant bacterial infections. In this study, erythroliposome (RM-PL), a biomimetic platform constructed by artificial and natural lipid membranes, was used to neutralize hemolytic PFTs. The detoxification ability of RM-PL was attributed to macrophages in the spleen and the liver, which absorbed and digested the toxins in lysosomes.
Anti-virulence strategy has been considered as one of the most promising approaches to combat drug-resistant bacterial infections. Pore-forming toxins (PFTs) are the largest class of bacterial toxins, inflicting their virulence effect through creating pores on the cell membrane. However, current solutions for eliminating PFTs are mostly designed based on their molecular structure, requiring customized design for different interactions. In the present study, we employed erythroliposome (denoted as RM-PL), a biomimetic platform constructed by artificial lipid membranes and natural erythrocyte membranes, to neutralize different hemolytic PFTs regardless of their molecular structure. When tested with model PFTs, including alpha-hemolysin, listeriolysin O, and streptolysin O, RM-PL could completely inhibit toxin-induced hemolysis in a concentration-dependent manner. In vivo studies further confirmed that RM-PL could efficiently neutralize various toxins and save animals' lives without causing damage to organs or tissues. In addition, we explored the underlying mechanisms of this efficient detoxification ability and found that it was mainly macrophages in the spleen and the liver that took up RM-PL-absorbed toxins through a variety of endocytosis pathways and digested them in lysosomes. In summary, the biomimetic RM-PL presented a promising system for broad-spectrum and powerful toxin neutralization with a mechanism of lysosome-mediated toxin degradation. (C) 2022 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
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