Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program

Bioactive materials harness the body's innate regenerative potential by directing endogenous progenitor cells to facilitate tissue repair. Dissolution products of inorganic biomaterials provide unique biomolecular signaling for tissue-specific differentiation. Inorganic ions (minerals) are vital to biological processes and play crucial roles in regulating gene expression patterns and directing cellular fate. However, mechanisms by which ionic dissolution products affect cellular differentiation are not well characterized. We demonstrate the role of the inorganic biomaterial synthetic two-dimensional nanosilicates and its ionic dissolution products on human mesenchymal stem cell differentiation. We use whole-transcriptome sequencing (RNA-sequencing) to characterize the contribution of nanosilicates and its ionic dissolution products on endochondral differentiation. Our study highlights the modulatory role of ions in stem cell transcriptome dynamics by regulating lineage-specific gene expression patterns. This work paves the way for leveraging biochemical characteristics of inorganic biomaterials to direct cellular processes and promote in situ tissue regeneration.

Automated summary

This study investigates the role of dissolution products of inorganic biomaterials in the differentiation of human mesenchymal stem cells, using whole-transcriptome sequencing to characterize their contribution. The findings demonstrate the modulatory role of ions in stem cell transcriptome dynamics, regulating lineage-specific gene expression patterns to direct cellular processes and promote tissue regeneration.