期刊
SCIENCE ADVANCES
卷 8, 期 8, 页码 -出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.abm5900
关键词
-
资金
- NIH RO1s [DK121200, DK108947]
- VA Merit Award [BX005322]
- American Heart Association Career Development Award [20CDA35320006]
- American Society of Nephrology Career Development Award
This study identifies circulating osteopontin (OPN) released from kidney tubule cells as a novel mediator in acute kidney injury (AKI)-induced acute lung injury (ALI). OPN triggers lung endothelial leakage, inflammation, and respiratory failure. The study also demonstrates the elevated levels of OPN in AKI patients and its correlation with kidney injury.
Tissue injury can drive secondary organ injury; however, mechanisms and mediators are not well understood. To identify interorgan cross-talk mediators, we used acute kidney injury (AKI)-induced acute lung injury (ALI) as a clinically important example. Using kidney and lung single-cell RNA sequencing after AKI in mice followed by ligand-receptor pairing analysis across organs, kidney ligands to lung receptors, we identify kidney-released circulating osteopontin (OPN) as a novel AKI-ALI mediator. OPN release from kidney tubule cells triggered lung endothelial leakage, inflammation, and respiratory failure. Pharmacological or genetic OPN inhibition prevented AKI-ALI. Transplantation of ischemic wt kidneys caused AKI-ALI, but not of ischemic OPN-global knockout kidneys, identifying kidney-released OPN as necessary interorgan signal to cause AKI-ALI. We show that OPN serum levels are elevated in patients with AKI and correlate with kidney injury. Our results demonstrate feasibility of using ligand-receptor analysis across organs to identify interorgan cross-talk mediators and may have important therapeutic implications in human AKI-ALI and multiorgan failure.
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