期刊
ACS SENSORS
卷 7, 期 5, 页码 1467-1475出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.2c00215
关键词
Biomimetic sensing; imprinted polymers; food safety; cell imprinting; graphene oxide
资金
- European Regional Development Fund through the AgrEU food project - Interreg VA Flanders-The Netherlands program [2014TC16RFCB046]
This work introduces a thermal biomimetic sensor based on imprinted polymer for the detection of Escherichia coli, utilizing graphene oxide (GO) functionalized polydimethylsiloxane (PDMS) films to enhance sensitivity. The concentration-dependent temperature change serves as a detection mechanism, achieving a detection limit of 80 +/- 10 CFU/mL with PDMS-GO imprints, showcasing its superior performance.
This work presents an imprinted polymer-based thermal biomimetic sensor for the detection ofEscherichia coli. A novel and facilebacteria imprinting protocol for polydimethylsiloxane (PDMS)films wasinvestigated, and these receptor layers were functionalized with grapheneoxide (GO) in order to improve the overall sensitivity of the sensor.Upon the recognition and binding of the target to the densely imprintedpolymers, a concentration-dependent measurable change in temperaturewas observed. The limit of detection attained for the sensor employingPDMS-GO imprints was 80 +/- 10 CFU/mL, a full order lower than neatPDMS imprints (670 +/- 140 CFU/mL), illustrating the beneficial effectof the dopant on the thermo-dynamical properties of the interfacial layer.A parallel benchmarking of the thermal sensor with a commercialimpedance analyzer was performed in order to prove the possibility ofusing the developed PDMS-GO receptors with multiple readout platforms. Moreover,S. aureus,C. sakazakiiand an additionalE. colistrain were employed as analogue species for the assessment of the selectivity of the device. Finally, because of the potential that thisbiomimetic platform possesses as a low-cost, rapid, and on-site tool for monitoringE. colicontamination in food safety applications,spiked fruit juice was analyzed as a real sample. Reproducible and sensitive results fulfill the limit requirements of the applicable European microbiological regulation
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