期刊
JOURNAL OF BIOTECHNOLOGY
卷 236, 期 -, 页码 166-175出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2016.08.015
关键词
Aqueous biphasic system; Ionic liquids; Adjuvants; Antibody; Extraction; Purification
资金
- CICECO-Aveiro Institute of Materials [FCT UID/CTM/50011/2013]
- national funds through the FCT/MEC
- FEDER
- FCT [SFRH/BD/92200/2013]
- European Research Council under the European Union's Seventh Framework Programme (FP7)/ERC [337753]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/92200/2013] Funding Source: FCT
Immunoglobulins G (IgG) could become widespread biopharmaceuticals if cost-efficient processes for their extraction and purification are available. In this work, aqueous biphasic systems (ABS) composed of polyethylene glycols and a buffered salt, and with ionic liquids (ILs) as adjuvants, have been studied as alternative extraction and purification platforms of IgG from a rabbit serum source. Eleven ILs were investigated to provide insights on the chemical features which maximize the IgG partitioning. It is shown that in polymer-salt systems pure IgG preferentially partitions to the polymer-rich phase; yet, the complete extraction was never attained. Remarkably, after the addition of 5 wt% of adequate ILs to polymer-salt ABS, the complete extraction of pure IgG in a single-step was accomplished. The best systems and conditions were then applied to the extraction and purification of IgG directly from rabbit serum samples. The complete extraction of IgG in a single-step was maintained while its purity in the polymer-rich phase was enhanced by ca. 37% as compared to the IL-free ABS. The antibody stability was also evaluated revealing that appropriate ILs are able to maintain the IgG stability and can be used as phase-forming components of ABS when envisaging the purification of high-cost biopharmaceuticals. (C) 2016 Elsevier B.V. All rights reserved.
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