期刊
FRONTIERS IN PEDIATRICS
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fped.2022.895074
关键词
early onset-inflammatory bowel disease; WES; monogenic diseases; consanguineous; digenic inheritance; gene expression
类别
资金
- Deputyship for Research and Innovation, Ministry of Education in Saudi Arabia [IFPIP: 542-140-1442]
- King Abdulaziz University, DSR, Jeddah, Saudi Arabia
This study identified the molecular basis of early onset inflammatory bowel disease (IBD) in Arab patients through whole exome sequencing. The ITGAV and FN1 genes were found to be associated with IBD, with abundant expression in the gastrointestinal tract and immune organs. The dysregulation of these genes disrupts immune homeostasis and may induce intestinal inflammation. These findings provide new insights into the molecular etiology of pediatric onset IBD.
BackgroundMolecular diagnosis of early onset inflammatory bowel disease (IBD) is very important for adopting suitable treatment strategies. Owing to the sparse data available, this study aims to identify the molecular basis of early onset IBD in Arab patients. MethodsA consanguineous Arab family with monozygotic twins presenting early onset IBD was screened by whole exome sequencing (WES). The variants functional characterization was performed by a series of computational biology methods. The IBD variants were further screened in in-house whole exome data of 100 Saudi cohorts ensure their rare prevalence in the population. ResultsGenetic screening has identified the digenic autosomal recessive mode of inheritance of ITGAV (G58V) and FN1 (G313V) variants in IBD twins with early onset IBD. Findings from pathogenicity predictions, stability and molecular dynamics have confirmed the deleterious nature of both variants on structural features of the corresponding proteins. Functional biology data suggested that both genes show abundant expression in gastrointestinal tract and immune organs, involved in immune cell restriction, regulation of different immune related pathways. Data from knockout mouse models for ITGAV gene has revealed that the dysregulated expression of this gene impacts intestinal immune homeostasis. The defective ITGAV and FN1 involved in integrin pathway, are likely to induce intestinal inflammation by disturbing immune homeostasis. ConclusionsOur findings provide novel insights into the molecular etiology of pediatric onset IBD and may likely pave way in developing genomic medicine.
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