期刊
JOURNAL OF BIOTECHNOLOGY
卷 218, 期 -, 页码 25-33出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2015.11.019
关键词
Haematococcus pluvialis; Photoautotrophic culture; Astaxanthin; Summer outdoor conditions; Red cyst cell (aplanospore) inoculation method; Iron(Fe2+) supplementation method
资金
- Energy Efficiency AMP
- Resources Technology RAMP
- D project of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant Korea government Ministry of Trade, Industry Energy (MOTIE) [20152010201900]
- National Research Foundation (NRF) grants [NRF-2013R1A2A1A01015644/2010-0027955]
- University-Institute Cooperation Program
- Korea CCS RAMP
- D Center (KCRC) grant - Korean Government Ministry of Science, ICT AMP
- Future Planning (MSIP) [2014M1A8A1049278]
The negative effect of heat stress on the autotrophic astaxanthin production by Haematococcus pluvialis has been observed during outdoor culture in summer. Under the summer conditions, the proliferation of vegetative cells was highly halted in the green stage and the inducibility in the biosynthesis of astaxanthin was partly hindered in the red stage. Herein, under outdoor summer conditions in which variations of the diurnal temperature occur, heat-stress-driven inefficient vegetative growth of H. pluvialis was highly improved by inoculating the red cyst cells; thereby, maintaining relatively moderate intracellular carotenoid levels in the green stage. Subsequently, a remarkably enhanced astaxanthin titer was successfully obtained by supplementing 50 mu M iron(II) to induce the heat stress-driven Haber-Weiss reaction in the red stage. As a result, the productivity of astaxanthin in the cells cultured under summer temperature conditions (23.4-33.5 degrees C) using the two methods of red cell (cyst) inoculation and the iron(Fe2+) supplementation was increased by 147% up to 5.53 mg/L day compared with that of the cells cultured under spring temperature conditions (17.5-27.3 degrees C). Our technical solutions will definitely improve the annual natural astaxanthin productivity in H. pluvialis in locations confronted by hot summer weather, particularly in large-scale closed photobioreactor systems. (C) 2015 Elsevier B.V. All rights reserved.
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