期刊
FRONTIERS IN GENETICS
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2022.868742
关键词
Hyriopsis schlegelii; Iso-seq; RNA-seq; gonad; 17 beta-hsd2
资金
- Chinese Ministry of Science and Technology through the National Key Research and Development Program of China [2018YFD0901400]
- China Agriculture Research System of Ministry of Finance (MOF)
- Ministry of Agriculture and Rural Affairs (MARA) [CARS-49]
This study combined Illumina RNA-seq and PacBio Isoform Sequencing to analyze the gonads of H. schlegelii and identified genes related to sex determination and gonadal development.
The freshwater mussel Hyriopsis schlegelii is a cultured bivalve in China, and the quality of the pearls produced is affected by the type of gonads. However, because of the lack of a published genome and the complexity of sex determination, research on sex reversal and development of this species is limited. In this study, Illumina RNA-seq and PacBio Isoform Sequencing (Iso-Seq) were combined to analyze the gonads of H. schlegelii. A total of 201,481 high-quality transcripts were generated. The study identified 7,922 differentially expressed genes in three comparison group (females versus males, hermaphrodites versus females, and hermaphrodites versus males). Twenty-four genes were identified as potential sex-related genes, including sox9 and wnt4 involved in sex determination, and vtg, cyp17a1 and 17 beta-hsd2 involved in gonadal development. We also speculated a possible pathways for the formation of hermaphroditism in H. schlegelii. The data provide a clear view of the transcriptome for H. schlegelii gonads and will be valuable in elucidating the mechanisms of gonad development.
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