期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 121, 期 5, 页码 517-522出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2015.09.005
关键词
Bacillus sp.; Immobilization; Purification; Solvent tolerant lipase; Transesterification
资金
- Ratchadaphiseksomphot Endowment Fund
- CU on the Frontier Research Energy Cluster
- Thailand Research Fund [IRG 5780008]
Lipase from Bacillus sp. isolated from the oil contaminated soil was purified by ammonium sulphate precipitation and ion-exchange chromatography with a 5.1-fold purification and 10.5% yield. SDS-PAGE analysis of the enzyme revealed the molecular mass of 24 kDa. The optimum pH and temperature for lipase activity were 6.5 and 37 degrees C, respectively. The isolated lipase was stimulated by pretreatment with methanol and ethanol as well as by divalent metal ions Ca2+, Mg2+ and Mn2+. The enzyme showed high activity towards oleic rich oils. The enzyme immobilized on celite could retain 90% lipase activity-after eight cycles. Transesterification of Botryococcus sp. oil using the immobilized enzyme for 40 h resulted in 80% yield of fatty acid methyl esters which had good properties for use as biodiesel. Overall results suggested that the solvent tolerant Bacillus lipase can be a potential biocatalyst for methyl ester production. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据