期刊
JOURNAL OF BIOPHOTONICS
卷 9, 期 9, 页码 948-957出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201500324
关键词
Microscopy; super-resolution; localization; PALM; STORM
资金
- UK Medical Research Council (MRC) [MR/K015834/1, MC_A652_SPz10]
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/M018423/1]
- Wellcome Trust [WT093465MA, WT095931/Z/11/Z]
- Institute of Chemical Biology Doctoral Training Centre - UK Engineering and Physical Science Research Council (EPSRC)
- Biotechnology and Biological Sciences Research Council [BB/M018423/1] Funding Source: researchfish
- Medical Research Council [MR/K015834/1, MC_UP_A652_1002, 1375542] Funding Source: researchfish
- BBSRC [BB/M018423/1] Funding Source: UKRI
- MRC [MC_UP_A652_1002, MR/K015834/1] Funding Source: UKRI
TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and TIRF taking advantage of multimode optical fibres and multimode diode lasers to provide the required excitation light. Combined with open source software and relatively simple protocols to prepare samples for STORM, including the use of Vectashield for non-TIRF imaging, this approach enables TIRF and STORM imaging of cells labelled with appropriate dyes or expressing suitable fluorescent proteins to become widely accessible at low cost.
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