期刊
FRONTIERS OF MEDICINE
卷 16, 期 2, 页码 216-226出版社
SPRINGER
DOI: 10.1007/s11684-022-0920-7
关键词
tsRNA; biomarker; hepatocarcinoma
资金
- Training Program of the Major Research Plan of the National Natural Science Foundation of China [92049109]
- National Natural Science Foundation of China [32000549, 82003024]
- Natural Science Foundation of Jiangsu Province [BK2020041989]
- Natural Science Foundation of Nanjing University of Chinese Medicine [NZY82003024]
This study identified tRF-Gln-TTG-006 as a potential biomarker for hepatocellular carcinoma (HCC) by analyzing tsRNA signatures in HCC serum. The tRF-Gln-TTG-006 signature showed high sensitivity and specificity in distinguishing HCC cases from healthy subjects, even in the early stage. In vitro studies confirmed the release of circulating tRF-Gln-TTG-006 from tumor cells and validated its biological function.
Hepatocellular carcinoma (HCC), which makes up the majority of liver cancer, is induced by the infection of hepatitis B/C virus. Biomarkers are needed to facilitate the early detection of HCC, which is often diagnosed too late for effective therapy. The tRNA-derived small RNAs (tsRNAs) play vital roles in tumorigenesis and are stable in circulation. However, the diagnostic values and biological functions of circulating tsRNAs, especially for HCC, are still unknown. In this study, we first utilized RNA sequencing followed by quantitative reverse-transcription PCR to analyze tsRNA signatures in HCC serum. We identified tRF-Gln-TTG-006, which was remarkably upregulated in HCC serum (training cohort: 24 HCC patients vs. 24 healthy controls). In the validation stage, we found that tRF-Gln-TTG-006 signature could distinguish HCC cases from healthy subjects with high sensitivity (80.4%) and specificity (79.4%) even in the early stage (Stage I: sensitivity, 79.0%; specificity, 74.8%; 155 healthy controls vs. 153 HCC patients from two cohorts). Moreover, in vitro studies indicated that circulating tRF-Gln-TTG-006 was released from tumor cells, and its biological function was predicted by bioinformatics assay and validated by colony formation and apoptosis assays. In summary, our study demonstrated that serum tsRNA signature may serve as a novel biomarker of HCC.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据