期刊
CRYSTALS
卷 12, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/cryst12050694
关键词
RNA; riboswitch; structure; X-ray crystallography; crystal packing; RNA binding; base pair
资金
- National Institutes of Health [GM118174, GM116632]
- National Institute of General Medical Sciences from the National Institutes of Health [P41 GM103403]
- NIH-ORIP HEI grant [S10OD021527]
- DOE Office of Science [DE-AC02-06CH11357]
This study demonstrates that tRNA fusion can simplify the crystallization and structure determination of RNA molecules. The approach was applied to the T-box riboswitch system, capturing the dynamic interaction between tRNA and the T-box antiterminator.
RNAs are prone to misfolding and are often more challenging to crystallize and phase than proteins. Here, we demonstrate that tRNA fusion can streamline the crystallization and structure determination of target RNA molecules. This strategy was applied to the T-box riboswitch system to capture a dynamic interaction between the tRNA 3 '-UCCA tail and the T-box antiterminator, which senses aminoacylation. We fused the T-box antiterminator domain to the tRNA anticodon arm to capture the intended interaction through crystal packing. This approach drastically improved the probability of crystallization and successful phasing. Multiple structure snapshots captured the antiterminator loop in an open conformation with some resemblance to that observed in the recent co-crystal structures of the full-length T box riboswitch-tRNA complex, which contrasts the resting, closed conformation antiterminator observed in an earlier NMR study. The anticipated tRNA acceptor-antiterminator interaction was captured in a low-resolution crystal structure. These structures combined with our previous success using prohead RNA-tRNA fusions demonstrates tRNA fusion is a powerful method in RNA structure determination.
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