期刊
CANADIAN JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
卷 2022, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2022/5332129
关键词
-
资金
- National Natural Science Foundation of China [81802777]
- Taishan Scholars Program of Shandong Province [Ts20190987]
- Shandong Higher Education Research Center Scientific Research Project [YJKT201953]
- Shandong Province 2018 Professional Degree Postgraduate Teaching Case Library Project [SDYAL18049]
- Shandong Province 2018 Postgraduate Mentoring Ability Improvement Project [SDYY18073]
- Qingdao Traditional Chinese Medicine Project [2021-zyym26]
- Clinical Medicine + X Project of Qingdao University Hospital.
This study found that lncRNA PVT1 promotes cell proliferation, invasion, and migration, and inhibits apoptosis and EMT process in gastric cancer through targeting YAP. The expression of PVT1 and YAP could be used for early detection of gastric cancer, and interfering with their interaction may inhibit the occurrence and development of gastric cancer.
Gastric cancer (GC) as a serious global health problem is a threat to human longevity. Plasmacytoma variant translocation 1 (PVT1) participates in the formation and progression of various cancers, including GC. The aim of this study is to investigate the mechanism underlying the functions of PVT1 and explore a novel target for the diagnosis and treatment of GC. Analysis of the TCGA dataset using the R software identified that the lncRNA PVT1 was greatly upregulated in GC tissues. Twenty pairs of GC and adjacent normal tissues were acquired from patients with GC, and the expression of PVT1 was evaluated using RT-qPCR. Furthermore, PVT1 expression was knocked down in GC cells using siRNA, and the GC cells were divided into control, negative control (NC), and siRNA groups. Cell proliferation ability was analyzed using Cell Counting Kit-8 (CCK8) and colony formation assays, whereas cell migration and invasion ability were investigated through wound healing and Transwell assays. Moreover, Western blotting was used to analyze the expression of Yes-associated protein (YAP) and epithelial-to-mesenchymal transition (EMT) proteins. We also found that PVT1 and YAP expressions were upregulated in the GC tissues compared with those in the adjacent nontumor tissues. Knockdown of PVT1 was found to inhibit the proliferation, invasion, and migration and promote apoptosis of GC cells. Furthermore, knockdown of PVT1 downregulated YAP and promoted phosphorylation of YAP, suggesting that PVT1 exerts actions on GC cells by targeting YAP and inhibits cell apoptosis in vitro. The EMT process was also inhibited by the knockdown of PVT1. In summary, lncRNA PVT1 facilitated cell proliferation, invasion, and migration and suppressed cell apoptosis by targeting YAP. This study suggests that the expressions of PVT1 and YAP could be used for the early detection of GC and the occurrence and development of GC could be inhibited by interfering the interaction of PVT1 and YAP, which will provide new insights for the diagnosis, treatment, and prognosis of GC.
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