期刊
BIOENGINEERED
卷 13, 期 4, 页码 10518-10539出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2022.2062526
关键词
Enzyme; immobilization; functionalized polymers; applications
资金
- Ministry of Higher Education, Malaysia [63933, 56051, UMT/CRIM/2-2/5 Jilid 2(10)]
- Universiti Malaysia Terengganu [UMT/CRIM/2-2/25/Jld.8 (58), 53381]
Enzymes of commercial importance have multifunctional features and are extensively used in various industries. Immobilizing enzymes on functionalized polymers and composites can enhance their stability and catalytic activity. Functionalized materials provide more functional groups to improve enzyme immobilization efficiency.
Enzymes of commercial importance, such as lipase, amylase, laccase, phytase, carbonic anhydrase, pectinase, maltase, glucose oxidase etc., show multifunctional features and have been extensively used in several fields including fine chemicals, environmental, pharmaceutical, cosmetics, energy, food industry, agriculture and nutraceutical etc. The deployment of biocatalyst in harsh industrial conditions has some limitations, such as poor stability. These drawbacks can be overcome by immobilizing the enzyme in order to boost the operational stability, catalytic activity along with facilitating the reuse of biocatalyst. Nowadays, functionalized polymers and composites have gained increasing attention as an innovative material for immobilizing the industrially important enzyme. The different types of polymeric materials and composites are pectin, agarose, cellulose, nanofibers, gelatin, and chitosan. The functionalization of these materials enhances the loading capacity of the enzyme by providing more functional groups to the polymeric material and hence enhancing the enzyme immobilization efficiency. However, appropriate coordination among the functionalized polymeric materials and enzymes of interest plays an important role in producing emerging biocatalysts with improved properties. The optimal coordination at a biological, physical, and chemical level is requisite to develop an industrial biocatalyst. Bio-catalysis has become vital aspect in pharmaceutical and chemical industries for synthesis of value-added chemicals. The present review describes the current advances in enzyme immobilization on functionalized polymers and composites. Furthermore, the applications of immobilized enzymes in various sectors including bioremediation, biosensor and biodiesel are also discussed.
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