Long non-coding RNA MEG3 promotes tumor necrosis factor-alpha induced oxidative stress and apoptosis in interstitial cells of cajal via targeting the microRNA-21/I-kappa-B-kinase beta axis

Interstitial Cells of Cajal (ICC) plays a critical role in the peristaltic contractions of the gastrointestinal and urinary tract. The dysfunction and loss of ICC contributes to hypokinetic disease, such as gallstoneand ureteropelvic junction obstruction . In the present study, we identified the underlying driving molecular signals of oxidative stress and apoptosis in ICC. ICC was isolated from small intestine of Balb/c mice, and stimulated with tumor necrosis factor-alpha (TNF-alpha). MTT and flow cytometry were performed to assess cell viability, apoptosis, and the level of reactive oxygen species in ICC, respectively. The level of malondialdehyde, superoxide dismutase, and glutathione peroxidase in cells were measured to assess oxidative stress. The expression of inflammatory factors (interleukin, IL-1 and IL-6) and apoptosis-related proteins were detected by western blot. We observed that TNF-alpha induced inflammation, oxidative stress and cell apoptosis in ICC. By using quantitative real-time PCR , we verified that the expression of long non-coding RNAMEG3 was elevated by TNF-alpha in ICC. Silencing MEG3 reversed inflammation, oxidative stress, and cell apoptosisin TNF-alpha-treated ICC. Subsequently, we confirmed that MEG3 sponged cytoprotective miR-21 to upregulate the expression of I-kappa-B-kinase beta (IKKB) and activate the nuclear factor kappa-B (NF-kappa B) pathway. Both miR-21 overexpression and IKKB knockdown reduced TNF-alpha-induced above symptoms in ICC. Taken together, we can conclude that MEG3 mediates inflammation, oxidative stress and apoptosis in TNF-alpha-treated ICC via the miR-21/IKKB-NF-kappa B axis. The study improves our understanding of the molecular mechanism of ICC reduction related diseases.

Automated summary

Interstitial Cells of Cajal (ICC) are crucial for peristaltic contractions in the gastrointestinal and urinary tract. This study investigates the molecular signals of oxidative stress and apoptosis in ICC induced by tumor necrosis factor-alpha (TNF-alpha). The results demonstrate that TNF-alpha induces inflammation, oxidative stress, and cell apoptosis in ICC. Additionally, MEG3 mediates these effects through the miR-21/IKKB-NF-kappa B axis. This study enhances our understanding of ICC-related diseases at the molecular level.