期刊
BIOENGINEERED
卷 13, 期 3, 页码 6590-6599出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2022.2042141
关键词
Apoptosis; artesunate; invasion; lung cancer; migration
The study found that artesunate has inhibitory effects on the proliferation, migration and invasion of non-small cell lung cancer cells, and induces apoptosis. This effect may be associated with decreased expressions of HuR and MMP-9 proteins.
We aimed to assess the effects of artesunate (ART) on the proliferation, migration, invasion and apoptosis of the non-small cell lung cancer cells A549 and H1299. The effects of ART and carboplatin (CBP) alone or in combination on the viability of A549 and H1299 cells were evaluated by MTT assay. The effects of 30 mu g/ml ART on cell invasion, migration and apoptosis were evaluated by Transwell assay, scratch assay and flow cytometry, respectively. The protein expressions of human antigen R (HuR) and MMP-9 after treatment with 30 mu g/ml ART for 48 h were detected by Western blotting. After 48 h of treatment, 9 mu g/ml ART in combination with 7 mu g/ml CBP exerted a mild synergistic effect on cell viability. The migration rates of cells treated with 30 mu g/ml ART and number of invasive cells were significantly lower, and the apoptosis rates were higher than those of the DMSO-treated group. HuR and MMP-9 expressions in cells treated with 30 mu g/ml ART for 48 h were significantly lower than those of the DMSO-treated group. ART suppresses the proliferation, migration and invasion of A549 and H1299 cells and induces their apoptosis, probably being associated with decreased expressions of HuR and MMP-9 proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据