期刊
APPLIED SCIENCES-BASEL
卷 12, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/app12073240
关键词
fertility; GPR30; medically assisted reproduction; pregnancy; sperm
类别
资金
- Fundacao para a Ciencia e a Tecnologia-FCT [2021.05487.BD, IFCT2015, PTDC/MEC-AND/28691/2017, CEECINST/00026/2018, UIDB/50006/2020, UIDB/00215/2020, UIDP/00215/2020, LA/P/0064/2020]
- Portuguese Society of Reproductive Medicine (SPMR)
- Fundação para a Ciência e a Tecnologia [UIDB/00215/2020, PTDC/MEC-AND/28691/2017, UIDP/00215/2020, 2021.05487.BD] Funding Source: FCT
The abundance of GPR30 mRNA in spermatozoa is not correlated with paternal BMI, age, or sperm quality parameters, but higher levels of GPR30 mRNA are associated with the achievement of biochemical pregnancy and clinical pregnancy in couples undergoing assisted reproduction treatments.
Estrogens, specifically 17 beta-estradiol (E2), play an important role in male health, including male fertility. The G protein-coupled receptor for estrogen 30 (GPR30) is essential for mediating the rapid non-genomic effects of E2 on a variety of testicular cells, including spermatozoa, although its molecular effects remain largely unknown. In this work, we hypothesized that the GPR30 mRNA abundance in spermatozoa could be correlated to sperm quality. Sperm GPR30 mRNA could also be carried into the oocyte, potentially impacting embryo development and the success of a pregnancy. For this study, 81 sperm samples were collected from couples seeking fertility treatment and undergoing medically assisted reproduction treatments (ART), following the World Health Organization guidelines. GPR30 mRNA abundance in spermatozoa was assessed with a quantitative polymerase chain reaction. The resulting data show that there is no correlation between the abundance of the GPR30 transcript with paternal BMI, age, or sperm quality parameters. Interestingly, we observed that higher levels of GPR30 mRNA abundance in spermatozoa were related to the achievement of biochemical pregnancy and clinical pregnancy (p < 0.05) by couples undergoing treatment. These results highlight the role of the sperm's RNA cargo in offspring development, suggesting that spermatozoa mRNA content can influence ART success.
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