4.6 Article

Genetic Diversity and DNA Fingerprinting in Broccoli Carrying Multiple Clubroot Resistance Genes Based on SSR Markers

期刊

APPLIED SCIENCES-BASEL
卷 12, 期 9, 页码 -

出版社

MDPI
DOI: 10.3390/app12094754

关键词

broccoli; genetic diversity; SSR markers; fingerprinting; clustering analysis

资金

  1. National Natural Science Foundation of China [32172580]
  2. China Agriculture Research System [CARS-23-A05]
  3. Agricultural Science and Technology Innovation Program [ASTIP]
  4. State Key Laboratory of Vegetable Germplasm Innovation [SKL-VGI]

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This study utilized SSR marker technology to perform DNA fingerprinting of 10 broccoli varieties, and successfully identified markers with good polymorphism to establish the fingerprinting of these varieties. This provides a scientific basis for the identification of multiple clubroot resistance genes in broccoli.
To identify cultivars quickly and accurately, DNA fingerprinting of 10 broccoli varieties was performed by using simple sequence repeat (SSR) marker technology. Highly informative and polymorphic SSR markers were screened using broccoli and rapeseed. Out of the 93 SSR marker pairs, 21 pairs were selected and found to have good polymorphism. Each marker pair generated 1 to 10 polymorphic bands with an average of 4.29. The average polymorphism information content (PIC) was 0.41 with a range from 0.16 to 0.95. Six selected marker pairs established the fingerprinting of the 10 accessions and their unique fingerprints. Cluster analysis of 10 accessions showed that the genetic similarity coefficient was between 0.57 and 0.91. They can be divided into 3 groups at the genetic similarity coefficient (GSC) of 0.73. The above results indicated that DNA fingerprinting could provide a scientific basis for the identification of broccoli polymerized multiple clubroot resistance genes. Research shows that SSR marker-based DNA fingerprinting further ensures plant seed purity.

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