Exogenous Selenium Treatment Promotes Glucosinolate and Glucoraphanin Accumulation in Broccoli by Activating Their Biosynthesis and Transport Pathways

Supplementation using selenium (Se) on plants is an effective and widely used approach. It can not only be converted to more Se rich compounds but promote the accumulation of glucosinolates (GSLs) with anti-carcinogenic properties. However, the molecular mechanism of Se in regulating GSLs synthesis remains unclear. In the present study, we analyzed the effects of Se treatment (50 mu M sodium selenite) on GSLs, glucoraphanin (4MSOB), and sulforaphane compounds in broccoli tissues. The transcript levels of genes involved in sulfur absorption and transport, GSLs biosynthesis, translocation, and degradation pathways were also evaluated. The study showed that Se treatment remarkably promoted the accumulation of total sulfur and total Se contents and increased Trp-derived GSLs levels in roots by 2 times. The 4MSOB concentration and sulforaphane content in fresh leaves was increased by 67% and 30% after Se treatment, respectively. For genes expressions, some genes involved in sulfate uptake and transporters, GSLs biosynthesis, and transporters were induced strongly upon Se exposure. Results revealed that exogenous Se treatment promotes the overaccumulation of GSLs and 4MSOB content in broccoli by activating the transcript levels of genes involved in sulfur absorption, GSLs biosynthesis, and translocation pathways.

Automated summary

Supplementation using selenium is an effective approach to promote the accumulation of glucosinolates with anti-carcinogenic properties in plants. However, the molecular mechanism of selenium in regulating glucosinolates synthesis remains unclear. This study showed that selenium treatment significantly increased the levels of total sulfur and total selenium contents, as well as Trp-derived glucosinolates levels in broccoli roots. The concentration of 4MSOB and sulforaphane content in fresh leaves also increased after selenium treatment. Moreover, the transcript levels of genes involved in sulfur absorption, glucosinolates biosynthesis, and transporters were induced upon selenium exposure.