期刊
APPLIED SCIENCES-BASEL
卷 12, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/app12084029
关键词
l-fucose isomerase; l-fucose; l-fuculose; extremophile; halothermophilic bacteria; Halothermothrix orenii
类别
资金
- Mid-career Researcher Program through the National Research Foundation of Korea (NRF) [2020R1A2B5B02002631]
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry - Ministry of Agriculture, Food, and Rural Affairs [32136-05-1-SB010]
- NRF [NRF-2020R1A6A3A03039153]
- Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety
- Korea University Grant
- National Research Foundation of Korea [2020R1A2B5B02002631] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study reports a l-FucI enzyme with both thermophilic and halophilic properties, which exhibited optimal activity at 50-60 degrees C, pH 7, in the presence of 0.5-1 M NaCl and 1 mM Mn2+ as a cofactor. This unique enzyme has potential applications in the biotechnological production of l-fucose.
l-Fucose isomerase (l-FucI)-mediated isomerization is a promising biotechnological approach for synthesizing various rare sugars of industrial significance, including l-fucose. Extremozymes that can retain their functional conformation under extreme conditions, such as high temperature and salinity, offer favorable applications in bioprocesses that accompany harsh conditions. To date, only one thermophilic l-FucI has been characterized for l-fucose synthesis. Here, we report l-FucI from Halothermothrix orenii (HoFucI) which exhibits both halophilic and thermophilic properties. When evaluated under various biochemical conditions, HoFucI exhibited optimal activities at 50-60 degrees C and pH 7 with 0.5-1 M NaCl in the presence of 1 mM Mn2+ as a cofactor. The results obtained here show a unique feature of HoFucI as a polyextremozyme, which facilitates the biotechnological production of l-fucose using this enzyme.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据