期刊
ADVANCED SCIENCE
卷 9, 期 20, 页码 -出版社
WILEY
DOI: 10.1002/advs.202200074
关键词
electrochemical detection; gold nanostructure; kidney organoid; organoid maturation; pluripotent stem cells
资金
- National Research Foundation of Korea (NRF) - Korean government (MSIT) [NRF-2019M3A9H2031820]
- Korean Fund for Regenerative Medicine - Ministry of Science and ICT
- Ministry of Health and Welfare [21B0601L1-01]
A new method is proposed for the real-time assessment of kidney organoids, allowing non-destructive evaluation of differentiation and maturation levels. This method utilizes electrochemical signals to differentiate kidney cells from off-target cells, providing a promising tool for organoid research.
Organoids that mimic the structural and cellular characteristics of kidneys in vitro have recently emerged as a promising source for biomedical research. However, uncontrollable cellular heterogeneity after differentiation often results in the generation of off-target cells, one of the most challenging issues in organoid research. This study proposes a new method that enables the real-time assessment of kidney organoids derived from stem cells. When placed on a conductive surface, these organoids generate unique electrochemical signals at approximate to 0.3 V with intensities proportional to the amount of kidney-specific cell types. Off-target cells (i.e., non-kidney cells) produce an electrical signature at 0 V that is distinguishable from other surrounding cell types, enabling non-destructive assessment of both the differentiation, and maturation levels of kidney organoids. The developed platform can be applied to other types of organoids and is thus highly promising as a tool for organoid-based drug screening, toxicity assessment, and therapeutics.
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