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Exosome Processing and Characterization Approaches for Research and Technology Development

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Methods for the physical characterization and quantification of extracellular vesicles in biological samples

Deborah L. M. Rupert et al.

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2017)

Review Spectroscopy

Mass spectrometry of extracellular vesicles

Gabriella Pocsfalvi et al.

MASS SPECTROMETRY REVIEWS (2016)

Article Environmental Sciences

Micro RNA in Exosomes from HIV-Infected Macrophages

William W. Roth et al.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH (2016)

Article Chemistry, Multidisciplinary

Integrated Magneto-Electrochemical Sensor for Exosome Analysis

Sangmoo Jeong et al.

ACS NANO (2016)

Review Pharmacology & Pharmacy

Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches

Dmitriy Khodakov et al.

ADVANCED DRUG DELIVERY REVIEWS (2016)

Article Chemistry, Analytical

A Stimuli-Responsive, Binary Reagent System for Rapid Isolation of Protein Biomarkers

Barrett J. Nehilla et al.

ANALYTICAL CHEMISTRY (2016)

Article Cell & Tissue Engineering

Elucidation of Exosome Migration Across the Blood-Brain Barrier Model In Vitro

Claire C. Chen et al.

CELLULAR AND MOLECULAR BIOENGINEERING (2016)

Review Biochemistry & Molecular Biology

Exosomes in the Pathology of Neurodegenerative Diseases

Jason Howitt et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2016)

Article Biochemistry & Molecular Biology

ExoCarta: A Web-Based Compendium of Exosomal Cargo

Shivakumar Keerthikumar et al.

JOURNAL OF MOLECULAR BIOLOGY (2016)

Article Biochemical Research Methods

Using Raman spectroscopy to characterize biological materials

Holly J. Butler et al.

NATURE PROTOCOLS (2016)

Article Biochemistry & Molecular Biology

Continuous polyethylene glycol precipitation of recombinant antibodies: Sequential precipitation and resolubilization

Nikolaus Hammerschmidt et al.

PROCESS BIOCHEMISTRY (2016)

Article Instruments & Instrumentation

Surface plasmon resonance microscopy: Achieving a quantitative optical response

Alexander W. Peterson et al.

REVIEW OF SCIENTIFIC INSTRUMENTS (2016)

Article Multidisciplinary Sciences

ExtraPEG: A Polyethylene Glycol-Based Method for Enrichment of Extracellular Vesicles

Mark A. Rider et al.

SCIENTIFIC REPORTS (2016)

Article Multidisciplinary Sciences

Real time and label free profiling of clinically relevant exosomes

Abu Ali Ibn Sina et al.

SCIENTIFIC REPORTS (2016)

Article Cell Biology

A novel multiplex bead-based platform highlights the diversity of extracellular vesicles

Nina Koliha et al.

JOURNAL OF EXTRACELLULAR VESICLES (2016)

Review Biochemistry & Molecular Biology

ESCRTs are everywhere

James H. Hurley

EMBO JOURNAL (2015)

Review Pathology

Nano-plasmonic exosome diagnostics

Hyungsoon Im et al.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2015)

Article Allergy

Exosome secretion by eosinophils: A possible role in asthma pathogenesis

Carla Mazzeo et al.

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY (2015)

Article Agriculture, Dairy & Animal Science

Bovine milk exosomes contain microRNA and mRNA and are taken up by human macrophages

Hirohisa Izumi et al.

JOURNAL OF DAIRY SCIENCE (2015)

Review Biochemical Research Methods

Mass-Spectrometry-Based Molecular Characterization of Extracellular Vesicles: Lipidomics and Proteomics

Simion Kreimer et al.

JOURNAL OF PROTEOME RESEARCH (2015)

Article Biochemical Research Methods

Exosome isolation: a microfluidic road-map

A. Liga et al.

LAB ON A CHIP (2015)

Article Biochemical Research Methods

Proteomics characterization of exosome cargo

Kevin L. Schey et al.

METHODS (2015)

Article Multidisciplinary Sciences

Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties

Xabier Osteikoetxea et al.

PLOS ONE (2015)

Review Cell Biology

Ectosomes and exosomes: shedding the confusion between extracellular vesicles

Emanuele Cocucci et al.

TRENDS IN CELL BIOLOGY (2015)

Review Cell Biology

An Apoptotic 'Eat Me' Signal: Phosphatidylserine Exposure

Katsumori Segawa et al.

TRENDS IN CELL BIOLOGY (2015)

Review Virology

Exosomes: Implications in HIV-1 Pathogenesis

Marisa N. Madison et al.

VIRUSES-BASEL (2015)

Article Multidisciplinary Sciences

Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol

Mikhail A. Livshts et al.

SCIENTIFIC REPORTS (2015)

Article Cell Biology

Optimized exosome isolation protocol for cell culture supernatant and human plasma

Richard J. Lobb et al.

JOURNAL OF EXTRACELLULAR VESICLES (2015)

Article Clinical Neurology

Plasma exosomal α-synuclein is likely CNS-derived and increased in Parkinson's disease

Min Shi et al.

ACTA NEUROPATHOLOGICA (2014)

Review Cell Biology

Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles

Marina Colombo et al.

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 30 (2014)

Review Biochemistry & Molecular Biology

Exosomes as new vesicular lipid transporters involved in cell-cell communication and various pathophysiologies

Michel Record et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS (2014)

Article Biochemistry & Molecular Biology

Lipid quantification method using FTIR spectroscopy applied on cancer cell extracts

Allison Derenne et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS (2014)

Review Biochemistry & Molecular Biology

Next generation sequencing technology: Advances and applications

H. P. J. Buermans et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2014)

Letter Cell Biology

Double-stranded DNA in exosomes: a novel biomarker in cancer detection

Caitlin Williams et al.

CELL RESEARCH (2014)

Article Medicine, Research & Experimental

Cardiac fibroblast-derived microRNA passenger strand-enriched exosomes mediate cardiomyocyte hypertrophy

Claudia Bang et al.

JOURNAL OF CLINICAL INVESTIGATION (2014)

Article Cardiac & Cardiovascular Systems

Cardiomyocytes mediate anti-angiogenesis in type 2 diabetic rats through the exosomal transfer of miR-320 into endothelial cells

Xiaohong Wang et al.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2014)

Review Biochemistry & Molecular Biology

MicroRNAs and HIV-1 Infection: Antiviral Activities and Beyond

Gokul Swaminathan et al.

JOURNAL OF MOLECULAR BIOLOGY (2014)

Article Biochemical Research Methods

Microfluidic device (ExoChip) for on-chip isolation, quantification and characterization of circulating exosomes

Shailender Singh Kanwar et al.

LAB ON A CHIP (2014)

Article Biotechnology & Applied Microbiology

Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor

Hyungsoon Im et al.

NATURE BIOTECHNOLOGY (2014)

Article Biology

Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers

Mu Li et al.

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES (2014)

Article Multidisciplinary Sciences

Quantitative and stoichiometric analysis of the microRNA content of exosomes

John R. Chevillet et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2014)

Review Immunology

Tetraspanins in extracellular vesicle formation and function

Zoraida Andreu et al.

FRONTIERS IN IMMUNOLOGY (2014)

Article Cell Biology

The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling

Jan Van Deun et al.

JOURNAL OF EXTRACELLULAR VESICLES (2014)

Article Cell Biology

Towards traceable size determination of extracellular vesicles

Zoltan Varga et al.

JOURNAL OF EXTRACELLULAR VESICLES (2014)

Article Cell Biology

Comparative analysis of discrete exosome fractions obtained by differential centrifugation

Dennis K. Jeppesen et al.

JOURNAL OF EXTRACELLULAR VESICLES (2014)

Article Cardiac & Cardiovascular Systems

Cardiac myocyte exosomes: stability, HSP60, and proteomics

Z. A. Malik et al.

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY (2013)

Article Biochemistry & Molecular Biology

Phospholipidomics reveals differences in glycerophosphoserine profiles of hypothermically stored red blood cells and microvesicles

Beatriz Bicalho et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2013)

Article Biochemistry & Molecular Biology

Molecular lipidomics of exosomes released by PC-3 prostate cancer cells

Alicia Llorente et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS (2013)

Review Oncology

Exosomes in cancer development, metastasis, and drug resistance: a comprehensive review

Asfar S. Azmi et al.

CANCER AND METASTASIS REVIEWS (2013)

Review Cell Biology

Extracellular vesicles: Exosomes, microvesicles, and friends

Graca Raposo et al.

JOURNAL OF CELL BIOLOGY (2013)

Article Biochemical Research Methods

Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles

Zongxing Wang et al.

LAB ON A CHIP (2013)

Review Endocrinology & Metabolism

A glimpse into past, present, and future DNA sequencing

Marcos Morey et al.

MOLECULAR GENETICS AND METABOLISM (2013)

Review Nanoscience & Nanotechnology

Characterization of magnetic nanoparticle by dynamic light scattering

JitKang Lim et al.

NANOSCALE RESEARCH LETTERS (2013)

Article Cell Biology

Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes

Rossella Crescitelli et al.

JOURNAL OF EXTRACELLULAR VESICLES (2013)

Article Cell Biology

How pure are your vesicles?

Jason Webber et al.

JOURNAL OF EXTRACELLULAR VESICLES (2013)

Review Biochemistry & Molecular Biology

Exosomes: Current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials

Alexander V. Vlassov et al.

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2012)

Article Biochemistry & Molecular Biology

Amedeo Avogadro's cry: What is 1 μg of exosomes?

Eugene D. Sverdlov

BIOESSAYS (2012)

Review Cardiac & Cardiovascular Systems

Non-cardiomyocyte microRNAs in heart failure

Anke J. Tijsen et al.

CARDIOVASCULAR RESEARCH (2012)

Article Cardiac & Cardiovascular Systems

Inhibition of miR-15 Protects Against Cardiac Ischemic Injury

Thomas G. Hullinger et al.

CIRCULATION RESEARCH (2012)

Article Cardiac & Cardiovascular Systems

Serum levels of microRNAs in patients with heart failure

Yaron Goren et al.

EUROPEAN JOURNAL OF HEART FAILURE (2012)

Article Biochemistry & Molecular Biology

Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy

Yi Lee et al.

HUMAN MOLECULAR GENETICS (2012)

Article Hematology

Single vs. swarm detection of microparticles and exosomes by flow cytometry

E. Van der Pol et al.

JOURNAL OF THROMBOSIS AND HAEMOSTASIS (2012)

Article Medicine, Research & Experimental

Exosome removal as a therapeutic adjuvant in cancer

Annette M. Marleau et al.

JOURNAL OF TRANSLATIONAL MEDICINE (2012)

Article Medicine, Research & Experimental

Identification and proteomic profiling of exosomes in human cerebrospinal fluid

Jonathan M. Street et al.

JOURNAL OF TRANSLATIONAL MEDICINE (2012)

Article Biochemical Research Methods

Characterization of Membrane-shed Microvesicles from Cytokine-stimulated β-Cells Using Proteomics Strategies

Giuseppe Palmisano et al.

MOLECULAR & CELLULAR PROTEOMICS (2012)

Editorial Material Biochemistry & Molecular Biology

Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation

Hina Kalra et al.

PLOS BIOLOGY (2012)

Article Cell Biology

Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes

Olivier G. de Jong et al.

JOURNAL OF EXTRACELLULAR VESICLES (2012)

Article Chemistry, Analytical

Quantitative Sizing of Nano/Microparticles with a Tunable Elastomeric Pore Sensor

Robert Vogel et al.

ANALYTICAL CHEMISTRY (2011)

Article Pharmacology & Pharmacy

Exosomes as intercellular signalosomes and pharmacological effectors

Michel Record et al.

BIOCHEMICAL PHARMACOLOGY (2011)

Review Immunology

The Roles of Tumor-Derived Exosomes in Cancer Pathogenesis

Chenjie Yang et al.

CLINICAL & DEVELOPMENTAL IMMUNOLOGY (2011)

Article Biochemistry & Molecular Biology

Lipid Raft Endocytosis and Exosomal Transport Facilitate Extracellular Trafficking of Annexin A2

Mallika Valapala et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2011)

Article Cell Biology

Selective transfer of exosomes from oligodendrocytes to microglia by macropinocytosis

Dirk Fitzner et al.

JOURNAL OF CELL SCIENCE (2011)

Article Nanoscience & Nanotechnology

Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis

Rebecca A. Dragovic et al.

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE (2011)

Article Neurosciences

Lysosomal dysfunction increases exosome-mediated alpha-synuclein release and transmission

Lydia Alvarez-Erviti et al.

NEUROBIOLOGY OF DISEASE (2011)

Article Dermatology

The immune reconstitution inflammatory syndrome

SanjayS Bosamiya

INDIAN JOURNAL OF DERMATOLOGY (2011)

Article Immunology

Genetic Characterization of HIV Type 1 Nef-Induced Vesicle Secretion

Syed A. Ali et al.

AIDS RESEARCH AND HUMAN RETROVIRUSES (2010)

Article Cardiac & Cardiovascular Systems

Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans

Guo-Kun Wang et al.

EUROPEAN HEART JOURNAL (2010)

Article Nanoscience & Nanotechnology

Separation of beta-human chorionic gonadotropin from fibrinogen using a MEMS size exclusion chromatography column

Yongmo Yang et al.

MICROFLUIDICS AND NANOFLUIDICS (2010)

Article Cell Biology

Rab27a and Rab27b control different steps of the exosome secretion pathway

Matias Ostrowski et al.

NATURE CELL BIOLOGY (2010)

Article Biochemistry & Molecular Biology

TI-VAMP/VAMP7 and VAMP3/cellubrevin: two v-SNARE proteins involved in specific steps of the autophagy/multivesicular body pathways

Claudio Marcelo Fader et al.

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2009)

Article Biochemistry & Molecular Biology

ARF6-Regulated Shedding of Tumor Cell-Derived Plasma Membrane Microvesicles

Vandhana Muralidharan-Chari et al.

CURRENT BIOLOGY (2009)

Article Cell Biology

Exosomes - vesicular carriers for intercellular communication

Mikael Simons et al.

CURRENT OPINION IN CELL BIOLOGY (2009)

Review Biochemistry & Molecular Biology

LRRK2 in Parkinson's disease: genetic and clinical studies from patients

Udhaya Kumari et al.

FEBS JOURNAL (2009)

Article Medicine, Research & Experimental

Paracrine induction of endothelium by tumor exosomes

Joshua L. Hood et al.

LABORATORY INVESTIGATION (2009)

Article Biochemistry & Molecular Biology

Quantitative miRNA expression analysis: Comparing microarrays with next-generation sequencing

Hanni Willenbrock et al.

Review Immunology

Membrane vesicles as conveyors of immune responses

Clotilde Thery et al.

NATURE REVIEWS IMMUNOLOGY (2009)

Article Chemistry, Analytical

High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets

Margaret Macris Kiss et al.

ANALYTICAL CHEMISTRY (2008)

Article Immunology

Human tumor-derived exosomes down-modulate NKG2D expression

Aled Clayton et al.

JOURNAL OF IMMUNOLOGY (2008)

Article Multidisciplinary Sciences

Ceramide triggers budding of exosome vesicles into multivesicular Endosomes

Katarina Trajkovic et al.

SCIENCE (2008)

Article Chemistry, Analytical

On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets

N. Reginald Beer et al.

ANALYTICAL CHEMISTRY (2007)

Article Chemistry, Multidisciplinary

Dual magnetic-/temperature-responsive nanoparticles for microfluidic separations and assays

James J. Lai et al.

LANGMUIR (2007)

Article Physiology

Rapid isolation of urinary exosomal biomarkers using a nanomembrane ultrafiltration concentrator

Anita Cheruvanky et al.

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY (2007)

Article Multidisciplinary Sciences

Alzheimer's disease β-amyloid peptides are released in association with exosomes

Lawrence Rajendran et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Review Biochemistry & Molecular Biology

Exosomes: A common pathway for a specialized function

Guillaume van Niel et al.

JOURNAL OF BIOCHEMISTRY (2006)

Article Cell Biology

Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane

AM Booth et al.

JOURNAL OF CELL BIOLOGY (2006)

Article Biochemistry & Molecular Biology

Raft ceramide in molecular medicine

E Gulbins et al.

ONCOGENE (2003)

Review Genetics & Heredity

Fundamentals of cDNA microarray data analysis

YF Leung et al.

TRENDS IN GENETICS (2003)