Electrospun Nanofiber Films Suppress InflammationIn VitroandEradicate Endodontic Bacterial Infection in an E. faecalis-Infected Ex Vivo Human Tooth Culture Model

Treatment failure of endodontic infections and their concurrent inflammations is commonly associated withmicrobial persistence and reinfection, also stemming from the anatomical restrictions of the root canal system. Aiming to address theshortcomings of current treatment options, a fast-disintegrating nanofibrousfilm was developed for the intracanal coadministrationof an antimicrobial (ZnO nanoparticles) and an anti-inflammatory (ketoprofen) agent. The electrospunfilms were fabricated basedon polymers that dissolve rapidly to constitute the actives readily available at the site of action, aiming to eliminate both microbialinfection and inflammation. The anti-inflammatory potency of the nanofiberfilms was assessed in anin vitromodel oflipopolysaccharide (LPS)-stimulated RAW 264.7 cells after confirming their biocompatibility in the same cell line. The nanofiberfilms were found effective againstEnterococcus faecalis, one of the most prominent pathogens inside the root canal space, bothin vitroandex vivousing a human tooth model experimentally infected withE. faecalis. The physical properties and antibacterial and anti-inflammatory potency of the proposed electrospun nanofiberfilms constitute a promising therapeutic module in the endodontictherapy of nonvital infected teeth. All manuscripts must be accompanied by an abstract. The abstract should briefly state the problemor purpose of the research, indicate the theoretical or experimental plan used, summarize the principalfindings, and point out majorconclusions.

Automated summary

A fast-disintegrating nanofibrous film was developed for the intracanal coadministration of antimicrobial and anti-inflammatory agents to address the treatment failure of endodontic infections. The film was found to eliminate microbial infection and inflammation, making it a promising therapeutic option for nonvital infected teeth in endodontic therapy.