期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 105, 期 6, 页码 1352-1360出版社
WILEY
DOI: 10.1002/jbm.b.33668
关键词
extracellular matrix; regenerative medicine; tissue engineering
资金
- Rosetrees Trust
- Stoneygate Trust
- Royal College of Surgeons of England Research Fellowship programme
- Irish Research Council for Science, Engineering and Technology (IRCSET)
- Marie Curie International Mobility Fellowship
- State Scholarships Foundation (IKY) of Greece
- Rosetrees Trust [M259-CD1] Funding Source: researchfish
Renal transplantation is well established as the optimal form of renal replacement therapy but is restricted by the limited pool of organs available for transplantation. The whole organ decellularisation approach is leading the way for a regenerative medicine solution towards bioengineered organ replacements. However, systematic preoptimization of both decellularization and recellularization parameters is essential prior to any potential clinical application and should be the next stage in the evolution of whole organ decellularization as a potential strategy for bioengineered organ replacements. Here we have systematically assessed two fundamental parameters (concentration and duration of perfusion) with regards to the effects of differing exposure to the most commonly used single decellularizing agent (sodium dodecyl sul-phate/SDS) in the perfusion decellularization process for whole rat kidney ECM bioscaffolds, with findings showing improved preservation of both structural and functional components of the whole kidney ECM bioscaffold. Whole kidney bioscaffolds based on our enhanced protocol were successfully recellularized with rat primary renal cells and mesenchymal stromal cells. These findings should be widely applicable to decellularized whole organ bioscaffolds and their optimization in the development of regenerated organ replacements for transplantation. (C) 2016 Wiley Periodicals, Inc.
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