期刊
3 BIOTECH
卷 12, 期 3, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-022-03114-5
关键词
Collagen type 1; Acid soluble extraction; Biophysical and bioactive characterisations; Tissue engineering applications
资金
- DSTINSPIRE Fellowship
In this study, collagen extracted from the skin of bony and cartilaginous fishes was compared in terms of their structural and bioactive properties. Silver carp skin collagen showed higher yield, stability, antioxidant activity, and better performance in terms of haemocompatibility, cell viability, and adhesion, indicating its potential commercial applications in tissue engineering, cosmetics, and food industries.
In this study, a comparative structural and bioactive analysis of collagen extracted from the skin of bony and cartilaginous fishes. The acid-soluble method was followed to extract the collagen from Hypophthalmichthys molitrix (silver carp) and Rhizoprionodon acutus (milk shark) followed by purification using Ion exchange chromatography. A higher yield of collagen was obtained from the skin of SCsk (69.45%) as compared to SHsk (55.29%). SDS PAGE displayed the characteristic alpha, beta bands of the collagen type1. The native conformation and secondary structure stability of collagen were confirmed by FTIR, XRD and CD studies. The SEM micrographs exhibited the layered and fibrillar nature of the collagen from SHsk and SCsk, respectively. Relative solubility and thermal denaturation analysis showed SCsk to be more stable, but SHsk could withstand higher temperatures. 53.65% of antioxidant activity was observed in SCsk collagen as compared to SHsk (45.9%). Haemocompatibility, cell viability and adhesion results also displayed silvercarp skin to be a better source than Shark skin collagen. The results establish the potential of silver carp collagen as a biomaterial that can have many commercial applications in tissue engineering, cosmetics and food industries.
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