期刊
TROPICAL PLANT BIOLOGY
卷 15, 期 2, 页码 148-156出版社
SPRINGER
DOI: 10.1007/s12042-022-09309-7
关键词
IbJOX4; IbWRKY1; IbSCF; IbEIN3-2; Upstream transcription factors; Yeast one-hybrid screening
资金
- Key-Area Research and Development Program of Guangdong Province [2020B020219001]
This study identified IbJOX4, IbWRKY1, IbSCF, and IbEIN3-2 as the upstream transcription factors of PIbMYB1-1 involved in anthocyanin biosynthesis in purple-fleshed sweet potato. The results revealed their important regulatory roles in anthocyanin synthesis and provided new insights into the potential mechanism and regulatory network in purple-fleshed sweet potato.
Transcription factors IbMYB1-1 had been identified involving anthocyanins biosynthesis in purple-flesh sweet potato. However, the upstream transcription factors on PIbMYB1-1 involed in anthocyanin biosynthesis had rarely been reported. In this study, the upstream transcription factors on PIbMYB1-1 were screened by yeast one-hybrid screening in purple-fleshed sweet potato storage roots. The results indicated that IbJOX4, IbWRKY1, IbSCF and IbEIN3-2 had been identified as the upstream transcription factors of PIbMYB1-1 to regulated anthocyanin biosynthesis in purple-fleshed sweet potato. The interaction between the promoters and upstream transcription factors were verified by yeast one-hybrid and luciferase reporter assays. Subcellular localization analysis indicated that IbWRKY1, IbSCF and IbEIN3-2 were nuclear proteins and functioned as transcription factors to regulate expression of downstream genes. IbWRKY1, IbSCF and IbEIN3-2 had been identified as the upstream transcription factors of PIbMYB1-1 involved in anthocyanin biosynthesis in purple-fleshed sweet potato. The results provided a possible underlying mechanism of anthocyanin biosynthesis and a potential understanding of the regulatory network in purple-fleshed sweet potato.
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