期刊
NANOMATERIALS
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/nano12060893
关键词
fluorinated ionic liquids; surface-active ionic liquids; human phenylalanine hydroxylase; phenylketonuria; encapsulation
类别
资金
- Associate Laboratory for Green Chemistry-LAQV [UID/QUI/50006/2020]
- Research Institute for Medicines
- iMed.Ulisboa [UIDB/04138/2020, UIDP/04138/2020]
- MOSTMICRO- ITQB grant - FCT/MCTES [UIDB/04612/2020, UIDP/04612/2020]
- FCT [PD/BD/128201/2016]
- Fundação para a Ciência e a Tecnologia [PD/BD/128201/2016] Funding Source: FCT
This study reveals that a biocompatible fluorinated ionic liquid can serve as a delivery system for human phenylalanine hydroxylase, increasing its enzymatic activity but impacting its thermostability.
Phenylketonuria (PKU) is an autosomal recessive disease caused by deficient activity of human phenylalanine hydroxylase (hPAH), which can lead to neurologic impairments in untreated patients. Although some therapies are already available for PKU, these are not without drawbacks. Enzyme-replacement therapy through the delivery of functional hPAH could be a promising strategy. In this work, biophysical methods were used to evaluate the potential of [N-1112(OH)][C4F9SO3], a biocompatible fluorinated ionic liquid (FIL), as a delivery system of hPAH. The results herein presented show that [N-1112(OH)][C4F9SO3] spontaneously forms micelles in a solution that can encapsulate hPAH. This FIL has no significant effect on the secondary structure of hPAH and is able to increase its enzymatic activity, despite the negative impact on protein thermostability. The influence of [N-1112(OH)][C4F9SO3] on the complex oligomerization equilibrium of hPAH was also assessed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据