4.7 Article

Impact of Fluorinated Ionic Liquids on Human Phenylalanine Hydroxylase-A Potential Drug Delivery System

期刊

NANOMATERIALS
卷 12, 期 6, 页码 -

出版社

MDPI
DOI: 10.3390/nano12060893

关键词

fluorinated ionic liquids; surface-active ionic liquids; human phenylalanine hydroxylase; phenylketonuria; encapsulation

资金

  1. Associate Laboratory for Green Chemistry-LAQV [UID/QUI/50006/2020]
  2. Research Institute for Medicines
  3. iMed.Ulisboa [UIDB/04138/2020, UIDP/04138/2020]
  4. MOSTMICRO- ITQB grant - FCT/MCTES [UIDB/04612/2020, UIDP/04612/2020]
  5. FCT [PD/BD/128201/2016]
  6. Fundação para a Ciência e a Tecnologia [PD/BD/128201/2016] Funding Source: FCT

向作者/读者索取更多资源

This study reveals that a biocompatible fluorinated ionic liquid can serve as a delivery system for human phenylalanine hydroxylase, increasing its enzymatic activity but impacting its thermostability.
Phenylketonuria (PKU) is an autosomal recessive disease caused by deficient activity of human phenylalanine hydroxylase (hPAH), which can lead to neurologic impairments in untreated patients. Although some therapies are already available for PKU, these are not without drawbacks. Enzyme-replacement therapy through the delivery of functional hPAH could be a promising strategy. In this work, biophysical methods were used to evaluate the potential of [N-1112(OH)][C4F9SO3], a biocompatible fluorinated ionic liquid (FIL), as a delivery system of hPAH. The results herein presented show that [N-1112(OH)][C4F9SO3] spontaneously forms micelles in a solution that can encapsulate hPAH. This FIL has no significant effect on the secondary structure of hPAH and is able to increase its enzymatic activity, despite the negative impact on protein thermostability. The influence of [N-1112(OH)][C4F9SO3] on the complex oligomerization equilibrium of hPAH was also assessed.

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