期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 105, 期 5, 页码 977-988出版社
WILEY
DOI: 10.1002/jbm.b.33632
关键词
reversible crosslink; vitreous substitute; in situ forming hydrogel; ECIS
资金
- NIH Core Grant [P30 EY02687]
- NIH Grant [EY021 620]
- Research to Prevent Blindness, Inc.
- Department of Veterans Affairs Rehab Merit Review [RX000657-01]
The natural vitreous is a biological hydrogel consisting primarily of a collagen and anionic hyaluronate. It is surgically removed in many ocular diseases and replaced with fluids, gases, or silicone oils. We have been interested in developing synthetic hydrogels as vitreous substitutes. In this study, we combined the stiffness and hydrophobicity of polymethacrylamide (PMAM) and the anionic nature of polymethacrylate (PMAA) to make copolymers that would mimic the natural vitreous. We used bis-methacryloyl cystamine (BMAC) to introduce thiol groups for reversible crosslink. The Mn of copolymers ranged from similar to 100 k to similar to 200 k Da (polydisperisty index of 1.47-2.63) and their composition as determined by titration, H-1 NMR and disulfide test were close to the feed ratio. The reactivities of monomers were as follows: MAM>MAA similar to BMAC. Copolymers with higher MAA contents gelled faster, swelled more, and had higher storage modulus (1.5 to 100 Pa) comparable to that of the natural vitreous. We evaluated the biocompatibility of copolymers by electric cell-substrate impedance sensing (ECIS) using human retinal pigment epithelial cells, primary porcine retinal pigmented epithelial cells, human microvascular endothelial cells adult dermis, and a fibroblast line 3T3. The biocompatibility decreases as the content of BMAC increases. (C) 2016 Wiley Periodicals, Inc.
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