期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 105, 期 1, 页码 319-328出版社
WILEY
DOI: 10.1002/jbm.a.35901
关键词
ECM hydrogels; dental stem cell delivery; spinal cord; bone; dentine
资金
- Universite Catholique de Louvain (F.S.R)
- International Foundation for Research in Paraplegia (IRP)
- Fonds National de la Recherche Scientifique (F.R.S.-FNRS)
- European Commission-Education, Audiovisual & Culture Executive Agency (EACEA)
Decellularized mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration. Bone, spinal cord, and dentine ECM hydrogels exhibited distinct structural, mechanical, and biological characteristics. All three hydrogels supported SCAP viability and proliferation. However, only spinal cord and bone derived hydrogels promoted the expression of neural lineage markers. The specific environment of ECM scaffolds significantly affected the differentiation of SCAP to a neural lineage, with stronger responses observed with spinal cord ECM hydrogels, suggesting that site-specific tissues are more likely to facilitate optimal stem cell behavior for constructive spinal cord regeneration. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 319-328, 2017.
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