Pb-Bearing Ferrihydrite Bioreduction and Secondary-Mineral Precipitation during Fe Redox Cycling

The significant accumulation of Pb from anthropogenic activities threatens environmental ecosystems. In the environment, iron oxides are one of the main carriers of Pb. Thus, the redox cycling of iron oxides, which is due to biotic and abiotic pathways, and which leads to their dissolution or transformation, controls the fate of Pb. However, a knowledge gap exists on the bioreduction in Pb-bearing ferrihydrites, secondary-mineral precipitation, and Pb partitioning during the bioreduction/oxidation/bioreduction cycle. In this study, Pb-bearing ferrihydrite (Fh_Pb) with various Pb/(Fe+Pb) molar ratios (i.e., 0, 2, and 5%) were incubated with the iron-reducing bacterium Shewanella oneidensis MR-1 for 7 days, oxidized for 7 days (atmospheric O-2), and bioreduced a second time for 7 days. Pb doping led to a drop in the rate and the extent of the reduction. Lepidocrocite (23-56%) and goethite (44-77%) formed during the first reduction period. Magnetite (72-84%) formed during the second reduction. The extremely-low-dissolved and bioavailable Pb concentrations were measured during the redox cycles, which indicates that the Pb significantly sorbed onto the minerals that were formed. Overall, this study highlights the influence of Pb and redox cycling on the bioreduction of Pb-bearing iron oxides, as well as on the nature of the secondary minerals that are formed.

Automated summary

This study investigates the changes in Pb-bearing ferrihydrite during bioreduction, finding that Pb doping leads to a decrease in reduction rate and extent, as well as influencing the formation of secondary minerals. Through redox cycling, Pb significantly sorbs onto the minerals that are formed.