期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
卷 104, 期 5, 页码 1090-1101出版社
WILEY
DOI: 10.1002/jbm.a.35644
关键词
biopolymers; crosslinking; mechanical properties; modulus; stiffness
资金
- Nanyang Technological University
- National Healthcare Group innovation seed grant [M4060911.706022]
Three dimensional (3D) alginate scaffolds with tunable mechanical and structural properties are explored for investigating the effect of the scaffold properties on stem cell behavior and extracellular matrix (ECM) formation. Varying concentrations of crosslinker (20-60%) are used to tune the stiffness, porosity, and the pore sizes of the scaffolds post-fabrication. Enhanced cell proliferation and adipogenesis occur in scaffolds with 3.52 +/- 0.59 kPa stiffness, 87.54 +/- 18.33% porosity and 68.33 +/- 0.88 m pore size. On the other hand, cells in scaffolds with stiffness greater than 11.61 +/- 1.74 kPa, porosity less than 71.98 +/- 6.25%, and pore size less than 64.15 +/- 4.34 m preferentially undergo osteogenesis. When cultured in differentiation media, adipose-derived stem cells (ASCs) undergoing terminal adipogenesis in 20% firming buffer (FB) scaffolds and osteogenesis in 40% and 60% FB scaffolds show the highest secretion of collagen as compared to other groups of scaffolds. Overall, this study demonstrates the three-way relationship between 3D scaffolds, ECM composition, and stem cell differentiation. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1090-1101, 2016.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据