Liver Transcriptome Response to Heat Stress in Beijing You Chickens and Guang Ming Broilers

Heat stress is one of the most prevalent issues in poultry production that reduces performance, robustness, and economic gains. Previous studies have demonstrated that native chickens are more tolerant of heat than commercial breeds. However, the underlying mechanisms of the heat tolerance observed in native chicken breeds remain unelucidated. Therefore, we performed a phenotypical, physiological, liver transcriptome comparative analysis and WGCNA in response to heat stress in one native (Beijing You, BY) and one commercial (Guang Ming, GM) chicken breed. The objective of this study was to evaluate the heat tolerance and identify the potential driver and hub genes related to heat stress in these two genetically distinct chicken breeds. In brief, 80 BY and 60 GM, 21 days old chickens were submitted to a heat stress experiment for 5 days (33 degrees C, 8 h/day). Each breed was divided into experimental groups of control (Ctl) and heat stress (HS). The results showed that BY chickens were less affected by heat stress and displayed reduced DEGs than GM chickens, 365 DEGs and 382 DEGs, respectively. The transcriptome analysis showed that BY chickens exhibited enriched pathways related to metabolism activity, meanwhile GM chickens' pathways were related to inflammatory reactions. CPT1A and ANGPTL4 for BY chickens, and HSP90B1 and HSPA5 for GM chickens were identified as potential candidate genes associated with HS. The WGCNA revealed TLR7, AR, BAG3 genes as hub genes, which could play an important role in HS. The results generated in this study provide valuable resources for studying liver transcriptome in response to heat stress in native and commercial chicken lines.

Automated summary

This study evaluated the heat tolerance and identified potential driver and hub genes related to heat stress in native and commercial chicken breeds through phenotypical, physiological, liver transcriptome comparative analysis and WGCNA. The results showed that native chickens were less affected by heat stress and had fewer differentially expressed genes (DEGs) related to metabolism activity, while commercial chickens had pathways related to inflammatory reactions. CPT1A and ANGPTL4 were identified as candidate genes associated with heat stress in native chickens, while HSP90B1 and HSPA5 were identified in commercial chickens. TLR7, AR, and BAG3 genes were revealed as hub genes playing an important role in heat stress.