期刊
FRONTIERS IN PHYSIOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2022.877133
关键词
Aphidifus gifuensis; transcriptome; odorant binding protein; spatial expression pattern; fluorescence binding assay
类别
资金
- National Natural Science Foundation of China [31801739]
- National Key Research and Development Program of China [2021YFE0115600, 2017YFD0201700]
- China's Donation to the CABI Development Fund
In this study, a comparative antennal transcriptomic analysis was conducted on Aphidius gifuensis to investigate the spatial expression pattern of odorant-binding proteins (OBPs) in different organs and tissues. The binding properties of two OBPs predominantly expressed in female legs were also studied. This research provides a foundation for further understanding the chemosensation in A. gifuensis.
In China, Aphidius gifuensis is one of the most common endoparasitoids of the green peach aphid Myzus persicae and grain aphid Sitobion miscanthi in the field. Insect odorant-binding proteins (OBPs) play vital roles in odor perception during feeding, host searching, mating and oviposition. In addition, some OBPs are involved in other physiological processes such as gustation and reproduction. In the present study, a comparative antennal transcriptomic analysis was applied between male and female A. gifuensis. The spatial expression patterns among antennae, heads, thoraxes, abdomens and legs of OBPs in both sexes were further profiled. Fifteen AgifOBPs were predicted, and 14 of them were identified by gene cloning, including 12 classic OBPs and 2 min-C OBPs. As expected, all OBPs were mainly expressed at high levels in antennae, heads or legs which are sensory organs and tissues. Finally, ligand binding properties of 2 OBPs (AgifOBP7 and AgifOBP9) were further evaluated. Female leg specifically expressed AgifOBP9 displays a broad and high binding property to aphid alarm pheromones, plant green volatiles and aphid sex pheromones (Ki < 10 mu M). However, female leg specifically expressed AgifOBP7 displays poor affinity for all tested ligands except CAU-II-11 ((E)-3,7-dimethylocta-2,6-dien-1-yl-2-hydroxy-3-methoxybenzoate), a reported (E)-beta-farnesene (EBF) analog with an exceptionally high binding affinity (Ki = 1.07 +/- 0.08 mu M). In summary, we reported the spatial expression pattern of the OBP repertoire in A. gifuensis, and further studied the binding properties of OBP7 and OBP9, which are mainly expressed in female legs, laying the foundation for the dissection of the contribution of OBPs to chemosensation in A. gifuensis.
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