Anti-Apoptosis of Podocytes and Pro-Apoptosis of Mesangial Cells for Telmisartan in Alleviating Diabetic Kidney Injury

Podocytes damage and mesangial cells expansion are two important pathological manifestations of glomerular injury in early diabetes. Telmisartan, as an angiotensin type 1 (AT(1)) receptor inhibitor, could improve advanced glycation end (AGE) products or angiotensin II (Ang II)-induced podocytes injury including detachment or apoptosis. In this current paper, we first confirmed the protective effect of telmisartan on early diabetic kidney injury in type 1 diabetic rats. Telmisartan reduced the loss of podocin and inhibited the expression of alpha-SMA, reflecting its protective effect on podocyte injury and mesangial proliferation, respectively. More interestingly we observed an opposite effect of telmisartan on the cell viability and apoptosis of podocytes and mesangial cells in a high-glucose environment in vitro. The anti-apoptotic effect of telmisartan on podocytes might be related to its inhibition of swiprosin-1 (a protein can mediate high glucose-induced podocyte apoptosis) expression. While telmisartan induced a high expression of PPAR gamma in mesangial cells, and GW9662 (a PPAR gamma antagonist) partially inhibited telmisartan-induced apoptosis and reduced viability of mesangial cells. In addition, high glucose-induced PKC beta 1/TGF beta 1 expression in mesangial cells could be blocked by telmisartan. These data provide a more precise cellular mechanism for revealing the protective effect of telmisartan in diabetic kidney injury.

Automated summary

Telmisartan can protect against early diabetic kidney injury by improving podocyte damage and mesangial cell proliferation. Interestingly, telmisartan has opposite effects on cell viability and apoptosis of podocytes and mesangial cells in a high-glucose environment. The anti-apoptotic effect of telmisartan on podocytes may be related to its inhibition of swiprosin-1 expression, while telmisartan induces high expression of PPARγ in mesangial cells and inhibits PKCβ1/TGFβ1 expression.