期刊
FRONTIERS IN PHARMACOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2022.806010
关键词
coffee extracts; chlorogenic acid; 5-CQA; macrophages; inflammation; immunomodulation; interferon-beta
资金
- PerkinElmer Inc
- Italian Ministry of Health Ricerca Corrente - IRCCS MultiMedica
- Italian Association for Cancer Research (AIRC MFAG) [22818]
- Cariplo Foundation [2019-1609]
- GIDRM
Research has shown that coffee extracts have anti-inflammatory properties and can inhibit the release of interferon-beta. The main component of coffee extracts, 5-O-caffeoylquinic acid, is believed to be responsible for the observed immunomodulatory effect.
The anti-inflammatory activity of coffee extracts is widely recognized and supported by experimental evidence, in both in vitro and in vivo settings, mainly murine models. Here, we investigated the immunomodulatory properties of coffee extracts from green (GCE) and medium-roasted (RCE) Coffea canephora beans in human macrophages. The biological effect of GCE and RCE was characterized in LPS-stimulated THP-1-derived human macrophages (TDM) as a model of inflammation. Results showed decreased amounts of TNF-alpha, IL-6 and IL-1 beta and a strong dose-dependent inhibition of interferon-beta (IFN-beta) release. Molecular mechanism of IFN-beta inhibition was further investigated by immunofluorescence confocal microscopy analysis that showed a diminished nuclear translocation of p-IRF-3, the main transcription factor responsible for IFN-beta synthesis. The inhibition of IFN-beta release by RCE and GCE was also confirmed in human primary CD14(+) monocytes-derived macrophages (MDM). The main component of coffee extracts, 5-O-caffeoylquinic acid (5-CQA) also inhibited IFN-beta production, through a mechanism occurring downstream to TLR4. Inhibition of IFN-beta release by coffee extracts parallels with the activity of their main phytochemical component, 5-CQA, thus suggesting that this compound is the main responsible for the immunomodulatory effect observed. The application of 5-CQA and coffee derived-phytoextracts to target interferonopathies and inflammation-related diseases could open new pharmacological and nutritional perspectives.
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