期刊
FRONTIERS IN PHARMACOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2022.871569
关键词
force measurement; contraction force; conformal coating; cardiac tissue model; in vitro model; human-induced pluripotent stem cell-derived cardiomyocytes; inotropic drug
资金
- Academy of Finland [310347, 310527]
- Finnish Cultural Foundation
- Pirkanmaa Regional fund [50211545]
- Academy of Finland (AKA) [310527, 310347, 310347, 310527] Funding Source: Academy of Finland (AKA)
This study developed a method for measuring cardiac contraction force using a dual-axis piezoelectric force sensor and human cell-based cardiac tissue constructs. The system was able to detect both positive and negative inotropic effects.
Contractility is one of the most crucial functions of the heart because it is directly related to the maintenance of blood perfusion throughout the body. Both increase and decrease in contractility may cause fatal consequences. Therefore, drug discovery would benefit greatly from reliable testing of candidate molecule effects on contractility capacity. In this study, we further developed a dual-axis piezoelectric force sensor together with our human cell-based vascularized cardiac tissue constructs for cardiac contraction force measurements. The capability to detect drug-induced inotropic effects was tested with a set of known positive and negative inotropic compounds of isoprenaline, milrinone, omecamtiv mecarbil, propranolol, or verapamil in different concentrations. Both positive and negative inotropic effects were measurable, showing that our cardiac contraction force measurement system including a piezoelectric cantilever sensor and a human cell-based cardiac tissue constructs has the potential to be used for testing of inotropic drug effects.
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