期刊
FRONTIERS IN PHARMACOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2022.827782
关键词
Scutellaria barbata D; Don; polysaccharides; purification; structure; anti-hepatoma activity
资金
- basic scientific research business fee project of Heilongjiang Provincial Department of Education [2018-KYYWFMY-0010]
- Heilongjiang scientific research project of traditional Chinese Medicine [ZHY18-1168, ZQG-056]
- innovative scientific research project for Postgraduates of Mudanjiang Medical University [YJSCX-MY16]
- Postdoctoral Scientific Research Startup Fund of Heilongjiang Province [LBH-Q15141]
This study investigated the optimal extraction conditions, structural characterization, and anti-hepatoma activity of acidic polysaccharides (SBP) extracted from Scutellaria barbata D. Don. The results showed that SBP-2A exhibited better anti-hepatoma activity compared to SBP-1A and SBP. In vitro experiments demonstrated that SBP-2A induced HepG2 cell death, apoptosis, and G1 phase blockage. Furthermore, SBP-2A inhibited tumor growth in H22 tumor-bearing mice. These findings suggest that SBP-2A may be a potential candidate for cancer prevention.
The Chinese medicinal herb Scutellaria barbata D. Don has antitumour effects and is used to treat liver cancer in the clinic. S. barbata polysaccharide (SBP), one of the main active components extracted from S. barbata D. Don, exhibits antitumour activity. However, there is still a lack of research on the extraction optimization, structural characterization, and anti-hepatoma activity of acidic polysaccharides from S. barbata D. Don. In this study, the optimal extraction conditions for SBP were determined by response surface methodology (RSM): the material-liquid ratio was 1:25, the extraction time was 2 h, and the extraction temperature was 90 degrees C. Under these conditions, the average extraction efficiency was 3.85 +/- 0.13%. Two water-soluble polysaccharides were isolated from S. barbata D. Don, namely, SBP-1A and SBP-2A, these homogeneous acidic polysaccharide components with average molecular weights of 1.15 x 10(5) Da and 1.4 x 10(5) Da, respectively, were obtained at high purity. The results showed that the monosaccharide constituents of the two components were fucose, galactosamine hydrochloride, rhamnose, arabinose, glucosamine hydrochloride, galactose, glucose, xylose, and mannose; the molar ratio of these constituents in SBP-1A was 0.6:0.3:0.6:30.6:3.3:38.4:16.1:8:1.4, and that in SBP-2A was 0.6:0.5:0.8:36.3:4.4:42.7:9.2:3.6:0.7. In addition, SBP-1A and SBP-2A contained uronic acid and beta-glucan, and the residue on the polysaccharide was mainly pyranose. The in vitro results showed that the anti-hepatoma activity of SBP-2A was better than that of SBP-1A and SBP. In addition, SBP-2A significantly enhanced HepG2 cell death, as cell viability was decreased, and SBP-2A induced HepG2 cell apoptosis and blocked the G1 phase. This phenomenon was coupled with the upregulated expression of P53 and Bax/Bcl-2 ratio, as well as the downregulated expression of the cell cycle-regulating protein cyclinD1, CDK4, and Bcl-2 in this study. Further analysis showed that 50 mg/kg SBP-2A inhibited the tumour growth in H22 tumour-bearing mice, with an average inhibition rate of 40.33%. Taken together, SBP-2A, isolated and purified from S. barbata showed good antitumour activity in vivo and in vitro, and SBP-2A may be a candidate drug for further evaluation in cancer prevention. This study provides insight for further research on the molecular mechanism of the anti-hepatoma activity of S. barbata polysaccharide.
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