期刊
JOURNAL OF BIOMATERIALS APPLICATIONS
卷 31, 期 6, 页码 923-932出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328216680343
关键词
Thermo-sensitive hydrogel; PLGA-PEG-PLGA; vaccine delivery system; intramuscular immunization; ovalbumin
资金
- Key Laboratory of Construction and Detection of Guangdong Province
- Natural Science Foundation of Guangdong Province [2014A03031, 2015A030313314]
- Science and Technology Project of Guangdong Province [2014A040401028]
In this work, we explored the potential of thermo-sensitive PLGA-PEG-PLGA with sol-gel transition temperature around 32 degrees C as an intramuscular vaccine delivery system by using ovalbumin as a model antigen. First, in vitro release test showed that the PLGA-PEG-PLGA-deriving hydrogels could release ovalbumin in vitro in a more sustainable way. From fluorescence living imaging, 50-200 mg/mL of PLGA-PEG-PLGA formulations could release antigen in a sustainable manner in vivo, suggesting that the PLGA-PEG-PLGA hydrogel worked as an antigen-depot. Further, the sustainable antigen release from the PLGA-PEG-PLGA hydrogels increased antigen availability in the spleens of the immunized mice. The intramuscular immunization results showed that 50-200 mg/mL of PLGA-PEG-PLGA formulations promoted significantly more potent antigen-specific IgG immune response. In addition, 200 mg/mL of PLGA-PEG-PLGA formulation significantly enhanced the secretion of both Th1 and Th2 cytokines. From in vitro splenocyte proliferation assay, 50-200 mg/mL of PLGA-PEG-PLGA formulations all initiated significantly higher splenocyte activation. These results indicate that the thermo-sensitive and injectable PLGA-PEG-PLGA hydrogels (particularly, 200 mg/mL of PLGA-PEG-PLGA-based hydrogel) own promising potential as an intramuscular vaccine delivery system.
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