Proteome Based Comparative Investigation of a High Sucrose Sugarcane Mutant in Contrast to the Low Sucrose Mother Variety by Using TMT Quantitative Proteomics

Sugarcane (Saccharum sp. hybrid) is a significant crop in the world's economy and fulfills 80% of the world's sugar production. To meet the rising sugar requirement, it is emphasized to enhance the cane sugar yield. In the present study, the TMT quantitative proteomic approach was applied to find the proteins associated with sucrose accumulation in a high sucrose sugarcane mutant GXB9 compared to its low sucrose mother variety B9. A total of 27,922 proteins were obtained, and 7484 proteins received functional annotations on blasting in COG, GO, NR, Pfam, and KEGG databases. A sum of 3,102 differentially expressed proteins (DEPs) was recognized when the immature and maturing internodes of GXB9 were compared with B9. Sucrose phosphate synthase (SPS), invertases (CWIN, NIN1, CINV2), and sucrose synthase (SuSy) are significant proteins involved in sucrose metabolism and accumulation in sugarcane. This study discovered that the DEPs of SPS (SPS2, SPS5) were upregulated, while SuSy, CWIN, NIN1, CINV2 were downregulated in the maturing internodes, and seven DEPs of trehalose phosphate synthase such as TPS6, TPS7, TPS9, TPS9, and trehalose phosphate phosphatase were downregulated in the immature and maturing internodes of GXB9 compared with B9. The result of our exploration would mediate progression in sugarcane varieties by concentrating on the manipulation of proteins associated with sucrose synthesis, metabolism, and accumulation through the use of advanced molecular approaches.

Automated summary

In this study, a proteomic approach was used to identify proteins associated with sucrose accumulation in a high sucrose sugarcane mutant. Several key proteins involved in sucrose metabolism were found, and the results provide insights for improving sugarcane varieties.