期刊
MICROMACHINES
卷 13, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/mi13030394
关键词
microchip electrophoresis; stray capacitance; capacitively coupled contactless conductivity detection; planar grounded electrode
类别
资金
- School of Mechanical Engineering of Guangxi University - Key-Area Research and Development Program of Guangdong Province [2019B020219003]
- Guangxi Bagui Scholars Project
This article proposes a novel design of a planar grounded capacitively coupled contactless conductivity detector (PG-C4D) based on a printed circuit board (PCB) to reduce the effect of stray capacitance. The effectiveness of the design has been verified through electrical field simulation and electrophoresis detection experiments. The PG-C4D with this new design shows improved sensitivity and repeatability, making it suitable for applications in agricultural and environmental monitoring.
In the microchip electrophoresis with capacitively coupled contactless conductivity detection, the stray capacitance of the detector causes high background noise, which seriously affects the sensitivity and stability of the detection system. To reduce the effect, a novel design of planar grounded capacitively coupled contactless conductivity detector (PG-C4D) based on printed circuit board (PCB) is proposed. The entire circuit plane except the sensing electrodes is covered by the ground electrode, greatly reducing the stray capacitance. The efficacy of the design has been verified by the electrical field simulation and the electrophoresis detection experiments of inorganic ions. The baseline intensity of the PG-C4D was less than 1/6 of that of the traditional C4D. The PG-C4D with the new design also demonstrated a good repeatability of migration time, peak area, and peak height (n = 5, relative standard deviation, RSD <= 0.3%, 3%, and 4%, respectively), and good linear coefficients within the range of 0.05-0.75 mM (R-2 >= 0.986). The detection sensitivity of K+, Na+, and Li+ reached 0.05, 0.1, and 0.1 mM respectively. Those results prove that the new design is an effective and economical approach which can improve sensitivity and repeatability of a PCB based PG-C4D, which indicate a great application potential in agricultural and environmental monitoring.
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