Multiphoton single-molecule localization by sequential excitation with light minima

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1-2 nm precision with moderate photon counts, enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.

Automated summary

Sequential excitation technique with minimal light enables precise localization of single fluorescent molecules with molecular resolution. Expanding this concept to multi-photon regimes can lead to even higher localization precision and deeper imaging in biological specimens.